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Micro Lab 5: Diff stains
Terms in this set (15)
A method to distinguish between different types of bacteria, often based on color differences
primary stain; impart color to all organisms in the smear
diluted iodine solution (gram's iodine) - second reagent
acts a mordant; a substance that enhances the union bw the dye and its substrate
Decolorizer - third reagent
dissolves and removes the primary stain from the cells (95% ethyl alcohol)
safranin - fourth reagent
counterstain; red dye
What is the most critical step?
What can the gram stain be affected by?
how vigorous/lightly the decolorization is & the age of culture- must be 24 hours or younger
what is more sensitive to dehydration by alcohol which will close up pores of their wall?
Which has a wall with higher lipid contents?
Gram -; lipid dissolved by alcohol, pores dont close
study gram stain steps
This set is often in folders with...
Lab 2: Handling/examining cultures
Micro Lab 6: diff stains II
Lab 7: Isolation of bacteria
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