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Micro Lab 6: diff stains II
Terms in this set (15)
hemophilus influenzae, neisseria meningitidis, E. Coli, Streptococcus pneumoniae, Klebsiella pneumoniae and group B streptococci have...
polysaccharide capsules on their surface
What reagents are used in a capsule stain?
Indian ink, loeffler's methylene blue
is a capsule stain a positive or negative stain?
What happens after reagents are added to capsule stain?
halos, colorless rings around cells
Who will develop endospores?
Bacillus, clostridium, sporosarcina
When does sporulation occur?
as the culture gets older
What are endospores resistant to?
What is used to penetrate endospore?
What reagents are used for the endospore stain?
melachite green (stains cell); safranin (red) - CHRISTMAS!!!! :)
When is the acid-fast stain used?
in application of medicine
What microorganisms are acid-fast?
mycobacterium; like M. tuberculosis, M. leprae
Acid-fast procedure uses initial treatment with carbon fuchsin followed by ____-____ and methylene blue
Acid-fast organisms are decolorized and thus appear....
non acid fast appears
how is the primary dye forced into the bacterial cell
by heating it to steaming
This set is often in folders with...
Lab 2: Handling/examining cultures
Micro Lab 5: Diff stains
Lab 7: Isolation of bacteria
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