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Micro Lab 12: Normal Flora of the Skin
Terms in this set (19)
Environment of the skin
dry, acidic pH
What microflora is usually on the skin?
Gram-positive bacteria (coagulase-negative staphylococci); anaerobic (in pores)
Why is gram-positive found on the skin?
they can withstand drying
Example of anaerobic bacteria living on skin
Propiobacterium acnes; metabolizes sebum, releasing fatty acids which enhance anti-microbial environment
Which 2 medias will be streaked for primary isolation?
mannitol salt agar (MSA) & staphylococcus medium
if the mannitol is fermented to produce acid, the phenol red in the medium changes from red to...
staphylococcus medium 110 (SM110) also contains NaCl and mannitol, but lack what color?
What is the advantage of using SM110 over MSA?
SM110 favors colony pigmentation by different strains of S. aureus
what will the color change of SM110 be?
no color change
Gram stains help differentiate what?
Staph from strep
Gram positive cocci are CATALASE positive may be either what?
micrococci or staphylococcus
appears as greenish zone around the colony- it is due to partial breakdown of RBCs
alpha hemolysis example
appears as a clear area around the colonies. It is due to complete lysis of the red blob cells
Strep. pyrogenes, Staph. aureus, all gram negative enteric bacteria
beta hemolysis examples
Staph. epidermis, Strep. faecalis
gamma hemolysis examples
THIS SET IS OFTEN IN FOLDERS WITH...
Lab 2: Handling/examining cultures
Micro Lab 5: Diff stains
Micro Lab 6: diff stains II
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