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Terms in this set (25)
What is the main purpose of a DNA probe?
hybridizes to a target sequence
The restriction site and cleavage pattern for the enzyme BamHI is pictured. Note the polarity of the sequence. What type of cleavage pattern is generated with respect to this sequence when cutting DNA with BamHI?
Sticky ends; 5' overhang
How many fragments would you expect to produce after cutting the sequence with
What would be the correct order of steps from the following list in performing a Southern blot?
i. Gel electrophoresis
ii. Incubation with labeled probe
iii. Hybridization of nucleic acids to membrane
iv. Digestion of DNA
v. Signal detection
iv, i, iii, ii, v
What restriction enzyme combination could you digest a sample of the plasmids with, so that when you run a side-by-side comparison (using gel electrophoresis) you would be able to definitively distinguish between plasmid A and plasmid B?
BglII and HindIII
While you are waiting on your gel to run, you are thinking about the way that different size fragments of DNA migrate through the agarose. You daydream a scenario where you use EcoRI, BglII and HindIII to digest plasmid B. What sizes would the fragments be in base pairs?
1200, 1000, 900, 300
You are a genetic counselor. You want to PCR amplify a region of the cystic fibrosis gene in order to determine the genotype of the prospective parents. The sequence of the flanking DNA is:
5' GGCTAAGATCTGAA.......................AATGTAGCGCCTT 3'
3' CCGATTCTAGACTT.......................TTACATCGCGGAA 5'
. 5'GGCTAAGATCTGAA and 5'AAGGCGCTACATT
Shown is a portion of the sequence trace from Sanger sequencing results that you have just received after your recent attempt at cloning (note that you will need to look at this in either colored print, or the PDF version on D2L). The colors in the trace correspond to nucleotides as follows: red=T, green=A, black=G, purple=C.
What is the sequence of the template?
start from left and put down opposite letters
In ex vivo gene therapy, _____.
therapeutic DNA is transfected into cells that are outside the human body; these cells are then returned to the patient
Most of the problems with past attempts at gene therapy are with _____.
the vector used to deliver the therapeutic gene
Select the option that lists two especially useful characteristics of cloning vectors.
high copy number and antibiotic resistance gene(s)
In the context of molecular genetics, reverse transcription PCR (RT-PCR) refers to
assembling a DNA sequence from an mRNA
When GMOs are generated through the transfer of genetic material across species, the GMO is said to be ________.
Gene therapy is ________.
an example of translational medicine
The CRISPR-Cas system of gene editing is based on what naturally occurring biological process?
bacterial defense against viral infection
When performing a sequencing reaction using Sanger sequencing, which ddNTPs must be included in the reaction?
all four ddNTPs must be present
Genomes from several primates have been sequenced and annotated. These primates range from chimpanzee to Rhesus monkey to Neanderthal and modern humans. What is the advantage of having so many primate sequences available to researchers?
Comparative genomics allows for determining evolutionary trends
Which nitrogenous bases are most likely to be methylated?
cytosines adjacent to guanines
Which statement about X inactivation is false?
All embryonic cells randomly inactivate paternal X chromosomes during early development.
Which of the following statements best characterizes mice pups that experienced low levels of maternal nurturing?
As adults they have increased promoter methylation
Which of these DNA sequences is palindromic when double-stranded?
In the polymerase chain reaction, the purpose of the steps in which the temp. is between 55C and 65C is
to allow the primers to anneal
What is the purpose of the dideoxynucleotides used in computer-automated DNA sequencing?
to cause defined chain termination and to label the synthesized DNA
You are interested in knowing whether the addition of estrogen to your cell culture induces expression of mRNA from your gene of interest. A good way to determine this effect is to use
Quantitative real-time PCR (qPCR)
Steps in correct order for a whole genome sequencing (WGS) strategy:
Shearing or digestion
Gene identification by bioinformatics
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