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artificial electron donor is used to reduce cytochrome C
colorless in reduced state
purple/blue when oxidized
collect bacteria from agar plate
place on blotter paper
apply reagent and observe color
most bacteria produce hydrogen peroxide, which is toxic to microbial cells. If the bacteria produces the enzyme catalase, which breaks down hydrogen peroxide into water and oxygen gas
Nitrase Reduction Test Purpose
to determine if an organism can reduce nitrate into nitrite using nitrate reductase
Nitrate Reductase outcome #2
NO3--->NO2--->other products (NH4, NO, N2, N2O)
nitrate reductase positive
No color change after Reagent C
no nitrate (NO3) present
nitrates were reduced to nitrites which were then reduced to other products
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