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Chapter 2: Tools of the Laboratory

Terms in this set (12)

T/F: "Culture" is defined as a visible accumulation of microorganisms in or on a nutrient medium.
True
Which terms describes the introduction of microorganisms into an environment in which they will multiply
inoculation
Different type of culture ___ provide nutrients fro microbial growth and can be designed to support the growth of certain microbes while inhibiting the growth of others.
media
Which of the following describes the process of placing a culture of microorganisms in a temperature-controlled chamber to encourage growth?
incubation
Microbial growth in a liquid broth medium is indicated by ___.
cloudiness, sediment, color change
Which of the following are used to inoculate bacterial culture?
Loops, Swabs
The polysaccharide found in sea algae and commonly used to prepare solid culture media is called ___.
agar
Which of the following statements regarding culturing microbes is true?
Some microbes found in nature can be grown on artificial medium but some cannot.
A discrete mound of cells growing on a solid nutrient surface is termed a(n) ___.
colony
The technique that involves spreading a small droplet of culture or sample over a solid medium with a sterile inoculating loop, resulting in a gradual dilution of the sample over the surface of the medium yielding isolated colonies, is known as the ___ plate method.
streak
If a dye settles around the boundary of the specimen rather than sticking to it, forming a bright silhouette of the microbe on a dark background, the image has been formed by ___ staining.
negative
A stain that requires the use of only one dye is a(n) ___ stain.
simple
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