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Ex. 15: Fungi
Terms in this set (19)
large group of eukaryotic, non-photosynthetic organisms that include
the yeasts, molds and fleshy fungi
multicellular filamentous organisms such as mildew, rusts and smuts
multicellular, filamentous organisms that produce a thick (fleshy) reproductive body
4 Methods of Culturing Fungi
- Slide Culture Method
- Cover Glass Method
- Giant Culture Method
- Scotch Tape Preparation
Media used to culture fungi
Sabouraud Dextrose Agar
Describe the Slide culture Method, Giant Culture, Cover Glass and Scotch Tape Preparation
Refer to Manual :)
How do you prepare the Moist culture chamber?
A culture chamber is prepared by placing 1-2 layers of filter paper on the bottom of a petri dish over which is fitted a U-shaped glass tubing that serves as the stage. Before the
slide culture is placed in the chamber, the filter paper is moistened with sterile water.
The fungal culture is incubated at what temperature?
Major disadvantage of direct examination using a coverslip
characteristic arrangement of the spores is disrupted when pressure is applied to the cover slip
Distinguishing characteristics of Fungi
- lack tissue differentiation
- cell wall made of chitin
- propagate by spores
Molds have microscopic filaments called? If the filament has crosswalls?
Hyphae; Septate hyphae
Mass of intermeshed hyphae
2 kinds of asexual spores seen in molds
Sporangiospores and Conidia
3 kinds of sexual spores?
Zygospores, Ascopores and Basidiospores
Multicellular structures found in yeasts
Only asexual spore produced by yeasts
Blastospore or Bud
The pH of Sabouraud Agar is adjusted to what pH? Why?
5.6; inhibit bacterial growth
Composition of SDA?
1% peptone, 4% glucose, 2% agar-agar
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