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Biology - LAB: Midterm
Terms in this set (74)
T/F: The sugar-phosphate groupings in DNA form the outside of the DNA double helix
T/F: Red light has more energy than green light
What is the standard metric unit for mass?
Which researcher was the first to isolate nucleic acids (nuclein) and destroy them?
In the "DNA Extraction from Fruit" laboratory, what is found in the cell lysate solution?
A soup of dissolved membranes, cellular proteins, DNA and other molecules
Removes chromosomes in association with the DNA
In the DNA Extraction from Fruit laboratory, what could be used to deproteinize the DNA solution?
Hydrogen peroxide was added to a piece of potato and potatoes contain the enzyme, catalase. Hydrogen peroxide was added to the potato and bubbles appeared to the presence of oxygen gas. If the hydrogen peroxide was removed and re-added to the potato, would bubbling occur again?
T/F: Electromagnetic energy consists of packets of energy called electrons that travel in waves
What is the correct order of steps of the scientific method?
Create a hypothesis
Test the hypothesis
Collect and analyze the data
What is the name of the device that selects the wavelength at which measurements are to be taken with the spectrophotometer?
When an enzyme is functioning at its optimal conditions, the enzyme:
causes the chemical reaction to proceed rapidly
What is the standard metric unit for length?
If a slide is moved to the right using the stage controls, what way does the image move?
What is found within the discussion of a scientific paper?
Significance of the work either in a related field or in future experiments
In the "DNA Extraction from Fruit" laboratory, what was the purpose of filtration in this lab?
What is the purpose of homogenization
It removes attached proteins from the DNA
In spectrophotmetry, optical density (OD) is the same as:
The ability to distinguish between two closely spaced, adjacent objects is a result of what part of the microscope?
What a beam of light passes through or exits the solution, the light that emerges from the liquid is called what type of light?
If the ocular lens has a magnification of 20x and total magnification of the image is 500x, then what is the magnification of the objective lens?
A given enzyme has an optimal pH of 3, which is:
Three different colored threads were crisscrossed on a slid. The different colored threads were then observed where they intersected on the scanning (4x) and low-power (10x) objective lens. What happens to the depth of focus when magnification was increased?
Group expected to have a positive result, allowing the researcher to show that the experimental set up was capable of producing results.
Which nitrogenous base is not found in DNA?
Convert cm to mm
multiply by 10
What type of microscope uses beams of electrons to provide a 3D view of the surface features of objects?
scanning electron microscope (SEM)
As magnification increases, the area of the object being viewed in the microscope:
T/F: The distance the objective lens is from the specimen when the specimen is in focus is called working distance
What was the optimal temperature for catalase in the "Enzyme Catalysis" laboratory?
37 degrees Celsius
What is not found in the abstract of a scientific paper?
visuals of the results
In the "Enzyme Catalysis" laboratory, what was the source of catalase?
What is the correct order of wavelengths of the visible light spectrum starting with the color with the longest wavelength?
red, orange, yellow, green, blue, indigo, violet
Curve in the upper surface of a liquid
Variables that are not changed
Depth of Field
Special distance from the lens where the specimen can be sharply focused
All of the gene's in an organism
The range of a pigment's ability to absorb various wavelengths of light.
In order to start a chemical reaction, the chemical reaction requires an input of energy to push the reactants over a barrier. This amount of energy is called:
When changing the wavelength on a spectrophotmeter, what is used to standardize the instrument?
T/F: The acknowledgements section of a scientific paper recognizes those who have played an active role in designing the experiment, carrying it out and/or analyzing data
Calculate reaction rate
What part of the spectrophotometer converts light energy into electrical energy?
Beer's law establishes a direct relationship between absorption and:
Holds the microscope slide
Lens yo look through, usually 10x power
Regulates the amount of light on the specimen
Adjustment focusing knob
Used to focus on speciment
Holds the eyepiece
What happens to the amount of light available when the magnification of the microscope is increased?
In the "DNA Extraction from Fruit" laboratory, by which process did cell lysis occur?
What is the purpose of the title page in a scientific paper?
It helps a reader to determine their initial interest in the content of the paper
Specimens viewed through the microscope appear
reversed (upside down) and inverted (backwards)
What are the sample holders used in the spectrophotometer called?
In the "DNA Extraction from Fruit" laboratory, what solution disrupted the cell and nuclear membrane?
Detergent and a salt-based lysis solution
Why does one see methyl orange as orange in color?
Methyl orange emits or reflects only the light of the orange wavelength.
When enzymes are placed into not optimal conditions, the enzyme's active site shape is altered and the enzyme loses its function. When this occurs, the enzyme is said to have:
DNA is tightly coiled around proteins (histones) that resemble a bead-like structure called a:
T/F: Enzymes are consumed in the chemical reaction and cannot be used repeatedly
What researchers described DNA as helical using X-ray diffraction studies?
James Watson and Francis Crick
T/F: Enzymes and substrates fit together like a lock-and-key therefore for every enzyme there is a binding site to fit a specific substrate
What is the incident beam?
Convert Celsius to Fahrenheit
F= C * 9/5 + 32
Convert Fahrenheit to Celsius
C = (F-32) 5/9
T/F: DNA is insoluble in ice-cold ethanol and precipitates or comes out of solution
When transferring 0.8mL of a chemical from its source to a test tube, what equipment would be most appropriate?
From the observation of the onion cells under the microscope, what structures are found in plant cells but not in animal cells?
Information describing color, odor, shape, or some other physical characteristic
Enzymes increase the rate of a chemical reaction by
decreasing the amount of activation energy needed
T/F: You do not need to completely refocus when changing between two parfocal lenses
A scientist is measuring blood glucose levels of two individuals over time. What type of graph would be best suited to display the data?
variable that is manipulated
The measurable effect, outcome, or response in which the research is interested.
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