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HW6 - Extrachromosomal Replication, F plasmid, & Conjugation
Terms in this set (22)
Tra locus: What is the name of the gene that regulates transcription, but does not encode a protein?
The F plasmid has 3 modes of DNA rep. Which one uses a rolling circle mechanism?
conjugation using oriT
F plasmid: Where is the nic site located?
F plasmid: How are the donor and recipient cells drawn together for the transfer of DNA during conjugation?
depolymerization of pilin protein in donor
Tra locus: Which protein(s) share(s) many of the activities of PhiX174 Protein A?
Why do Hfr cells promote a high level of recombination when added to a F minus population of bacteria?
The F plasmid often does not transfer to the recipient, but chromosomal DNA does. Homologous recombination occurs between this newly introduced DNA and the recipient chromosome.
F plasmid immunity: Which inner membrane protein blocks the transfer of DNA between F positive cells?
Tra locus: Which protein is the first protein translated on the long polycistronic transcript (mRNA) and recognizes oriT?
Tra locus: Which protein is a positive transcriptional regulator and controls expression or TraM and TraY/I?
Tra locus: Which protein is called the "pilot" protein because it covalently links to the 5' end of the transferred DNA and leads the DNA into the recipient cell?
Which term below signifies a free F plasmid containing a portion of the host chromosome?
Helicase activity: How fast does the TraY/I complex unwind DNA starting at oriT?
1,200 bp per second
Problem: An F plasmid contains an insertion sequence (IS element) that is identical to one found on the host chromosome. However, the IS element copy on the F plasmid contains a defective transposase coding region (a single bp mutation).
It will use homologous recombination to integrate into the chromosome at the site of the IS element.
Tra locus: Which protein directs the TraY/I/M-DNA complex to the T4SS channel? (Note: It was not covered in the lectures, but TraT/Y complex also contains TraM.)
Which term below signifies a cell containing a free F plasmid?
F plasmid immunity: Which protein blocks contact between the Tip protein of the pilus between two F+ cells?
When a portion of the bacterial chromosome is transferred to the donor cell from an Hfr donor, how does this DNA integrate into the recipient chromosome?
double homologous recombination between the new fragment and the host chromosome
If an insertion sequence (IS element) was located on the E. coli genome half-way around the circle from an integrated copy of the F plasmid, how long would it take for the insertion sequence to transfer to the recipient cell?
How long would it take for an integrated copy of the F plasmid to replicate itself by conjugation under standard lab conditions?
a little more than 100 mins
Tra locus: Which protein(s) sense that a stable mating pair has formed and then allows TraI to nick at oriT?
When Hfr cells are mixed with F minus cells the recipient cell acquire traits found in the Hfr strain. Why are the recipients usually still F minus?
It takes a long time to transfer the F plasmid, since the host chromosomal DNA is transferred first. Usually the mating is disrupted before F has a chance to transfer itself.
When the F plasmid integrates into the chromosome, which origin does it use to replicate?
oriC, and its own oriV is suppressed
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