Mol bio me

Monomeric proteins do not contain a
A. Primary structure
B. Secondary structure
C. Tertiary structure
D. Quaternary structure
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Terms in this set (26)
Monomeric proteins do not contain a
A. Primary structure
B. Secondary structure
C. Tertiary structure
D. Quaternary structure
D) Quaternary structure
A recombination DNA vector:
A. Cannot replicate and I host
B. Does not contain bacterial DNA sequences
C. Contains DNA sequences from only one source
D. Contains DNA sequences that can be cleaved by restriction enzymes
D. Contains DNA that can be cleaved by restriction enzymes
A polylinker increases the versatility of a DNA plasmid because it contains:
A. A DNA recognition sequence for several different restriction enzymes
B. A DNA sequence to allow the vector to replicate
C. A DNA sequence that confers resistance to ampicillin
D. b and c
A!
Next Gen Sequencing is much more efficient than Sanger because:
A. It uses RNA template instead of DNA
B. It uses gel electrophoresis to resolve end-labeled strands of DNA
C. It uses PCR amplification
D. It uses PCR and gel
C!
Protein secondary structure elements such as alpha helixes and beta sheets constitute major folding patterns in proteins. What is true
Folding patterns result from H bond between carboxyl and N-O groups in polypeptide bonds
Which of the following can be a function for intrinsically disordered Protein sequences?
A high specificity binding to other proteins
B Cell signaling through covalent modification of the protein sequence
C tethering to hold interacting proteins in close proximity
D Formation of a diffusion barrier from a dance network of such sequences
E All of the above
E!
Phosphorylation of a protein by a protein kinase
A. Adds two positive charges to the protein
B. Activates the protein
C. Deactivates the protein
D. Can create a binding site for the other proteins
E. Requires the hydrolysis of two molecules of ATP per phosphorylated residue
D!
protein self-splicing is..
Auto catalytic!
Size of the haploid human genome
3,300 kb
Barr body formation is an example of an —- process in which heritable changes affecting gene expression are not encoded by the DNA sequence
Epigenetics
3 things that make us believe mitochondria where bacteriaTheir size 1x2 ; cardiolipin which is found in bacteria; tightly packed and circular DNA without intronstelemoraseExtends the length of linear chromosomesClosed compact form of chromatinHeterochromatinThe open form of chromatinEuchromatinHeteroplasmyA mixture of mutant and wild type dnaTheory that proposes modern mitochondria came from respiratory active bacteriaEndosymbiotic theoryThe largest group of transposable SINE elementsAlu 1,100,000In nucleosomes at the centromere histone H3 is replaced byCENP-ABecause of truncations the average size of a human LINE element is900bpLINE's areNon LTR retrotansposonsWhat do you need to replicate a circular chromosomecentromeres, replication origin, TelomeresLysine methylationLeads to a more condensed structure due to the stabilization of the positive lysine chargeLysine AcetylationNeutralizes the positive charge of lysine and leads to a more open structurePhosphorylationLeads to a more open structure due to the addition of a negative charge repelling DNA and its negative chargeUbiquitinationWhen a large molecule up to 76bp added to the lysine doesn't allow for condensing and leads to a more open structureMicro satellites are known asShort tandem repeats