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Terms in this set (13)
Explain why genetic clones may not be exactly the same?
Differences in gene expression (penetrance) Differences in methylation (epigenetics)
What technique could we use if we wanted to try and determine if an outbreak of food poisoning was from a known strain of E. coli 0157H7 that we don't know the sequence for? (2 points)
Why is it less work to sequence using fluorescent chain terminators rather than radioactive chain terminators?
1 reaction instead of 4
What are two advantages of using deep sequencing over using fluorescent chain terminators?
Examine individual sequences instead of a consensus sequence and results are in real- time
If you only know a short bit of the sequence of your target bacterial gene, but have unlimited amounts of genomic DNA and want to determine the sequence of the promoter what technique could you use?
Partial credit: shotgun sequencing
If you want to enzymatically cut up genomic DNA into fragments of approximately 300 bp in length, which enzyme would you use? (1 point). Explain you answer. (1 point)
BstU I Frequency of cutting expected to be every 44 or 286 bases
What is the advantage of using satellites (micro or mini) compared to SNPs or indels to identify individuals or specific populations?
Frequently different enough to utilize (SNPs and indels too rare)
What typically causes the formation of satellites (micro or mini)?
When testing for genetic diseases, what is the difference between a linked gene and an associated gene?
Linked = cause of genetic disease (ex CF, sickle cell etc)
Associated = increase likelihood of disease but not the direct cause (ex cancer)
What are two advantages of using reporter genes to monitor gene expression? (2 point) What is a disadvantage of using reporter genes to monitor gene expression? (1 point)
Determine presence or absence but also quantify amounts
Have to use genetic engineering to add reporter gene to gene of interest
Based on what you learned in class, list four reasons why we might want to genotype individuals. (2 points)
Paternity/maternity testing Genetic disease
Organ/tissue transplant Genetic ancestry
What could you do if you wanted to determine which promoters in bacteria are activated (turned on) under a change of conditions (perhaps an increase in temperature)?
Describe how an array can be used to either detect changes in gene expression or detect SNPs.
A platform (slide) has different spots to which specific sequences are placed. For SNPs you detect if a sequence is bound to or not (whether SNP is present or not) and is detect by a fluorescent signal at that spot. For gene expression you detect if a sequence is bound more (up regulated) or less (down regulated) under a second condition.
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