uses optical modifications to exaggerate differences in density, making image appear almost 3-D
(light microscopy) fluorescence
shows the locations of specific molecules in the cell by tagging the molecules with fluorescent dyes or antibodies, which then absorb UV radiation and emit visible light
(light microscopy) confocal
a fluorescent optical sectioning technique that uses a pinhole aperture to eliminate out of focus light from a thick sample, creating a single plane of fluorescence in the image
refers to the cellular anatomy revealed by an electron microscope
(electron microscopy) scanning electron microscope
shows a 3-D image of the surface of a specimen
(electron microscopy) transmission electron microscopy
profiles a thin section of a specimen, revealing its ultrastructure
Disadvantages of electron microscopy
the methods used to prepare the specimen kill the cells and specimen preparation can introduce artifacts, structural features seen in micrographs that do not exist in the living cell
scanning electron micrograph
transmission electron micrograph
the study of cell structure
the study of the molecules and chemical processes (metabolism) of cells
technique for studying cell structure and function, which takes cells apart and separates the major organelles and other subcellular structures from one another
How do the stains used for light microscopy compare to those for electron microscopy?
Stains used for light microscopy are colored molecules that bind to cell components, affecting the light passing through, while stains used for electron microscopy involve heavy metals that affect the beams of electrons passing through
Which type of microscope would you use to study the changes in shape of a living white blood cell?
Which type of microscope would you use to study the details of surface texture of a hair?
Which type of microscope would you use to study the detailed structure of an organelle?