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7 terms

# 5 Endospore Stain

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Endospore stain
differential stain which stains endospore or spore bacteria(Clostridium and Bacillis)
Spore
refers to a structure that exits free of the mother cell. Highly resistant to hostile chemical and physical conditions. Tough outer covering composed primarily of Keratin. Can go into suspended animation.
endospores
refers to a a spore structure contained within mother cell. My be located in the middle of the cell (central0, the end (terminal), or between the end and the middle (subterminal)may be round or oval
primary stain
malachite green, stains both vegetative cells and endospores. Heat is required to penetrate endospores
decolorizer
water. Selective loosens malachite green from the vegetative cells but not endospores
counter stain
safranin. Stains the decolorized vegetative cells pink. Resulting in vegetative pink cells and endospore cells green or clear.
procedure
1) air dry-heat fix 2) steam -5 mins.3)paper towel 4) malachite green - 2 min. - rinse 5) safranin- 30 sec. rinse