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Microbiology Lab Quiz 4
Terms in this set (30)
areas where virus-infected cells have been destroyed and show up as a clear, well-defined patches in the cell sheet
a phage that specifically attacks E. coli.
A virus that infects bacteria
obligate intracellular parasites
cannot multiply unless they invade a specific host cell and instruct its genetic and metabolic machinery to make and release new viruses
a viral reproductive cycle in which copies of a virus are made within a host cell, which then bursts open, releasing new viruses
a viral reproductive cycle in which the viral DNA is added to the host cell's DNA and is copied along with the host cell's DNA
why is additional e. coli B added later after first incubation?
to have it as a standard of where it came from
tryptone soft agar purpose
nonselective and allow a wide array of bacteria to grow
how to calculate VCN
378 * 1/10^-3
378 * 10^3
vector copy numbers; CF/mL
formula for VCN
Calculate dilution factors from a set of tubes
why are eskape organisms not used in tiny earth project?
too dangerous to use
incubate at 30 degree celcius?
most eskape pathogen grow at this temperature
safe relative to eskape pathogens?
safe to use in lab
ESKAPe safe relatives?
prevent fungus growth. antifungal agents that is too toxic to humans
termed antiboitics. discovered streptomyces from grises. It was a cure for TB
chemotherapy. magic bullet
discovery of cyclohexamide
how does antibiotic resistance arise?
spontaneous mutation or acquired resistance gene
characteristic of antibiotic?
spectrum of activity
shouldn't eliminate normal microbiota
specific in action
inhibition of cell wall synthesis?
penicillin, bacitracin, cephalosporins, vancomycin
petty boys cause violence
inhibition of protein synthesis?
tetracycline, erythromycin, azithromycin)
inhibition of DNA and RNA?
Injury to plasma membrane?
Inhibition of essential metabolites?
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