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Biotech Chapter 5: Plasmids and Transformation Quiz (Original + Quizmass Sem. 1)
Terms in this set (54)
Ideal prokaryotic vector is a
Small circular pieces of DNA that can be transferred between bacteria are classified as
If DNA is stored or replicated it is called a _________ vector
If DNA is used to produce a product it is called an _________ vector
Process in which bacteria pick up DNA from the environment is called
Bacteria in the _______ phase are most capable to pick up plasmids
Exposing the cell membrane to the chemical _______ ________ makes it more competent
calcium chloride (CaCl2)
The process of ______ shock opens up the pores to increase efficiency
The process of ______ shock closes the pores trapping in the plasmids
Eukaryotic cells are transformed/ transfected by using an electrical current through the process of
Electroporation uses a structure known as a ________ plasmid
Plasmids are opened with a _________ enzyme (Ex: _______)
restriction; Pst1, EcoR1, or HindiII
The gene of interest is inserted because it has the same ________ ends since it was cut with the same enzyme
Insertion is made permanent with the enzyme ____ ____ which joins the ends together.
Purification of plasmids from a digested bacterium is first done with _______ to separate cells parts by mass with the final step in purification using ice cold _______ to precipitate the plasmid in the supernatant
Site that signifies the beginning of replication
A/an ________ site where the gene of interest is inserted
A/an _______ _______ gene is inserted so that the bacteria can be grow on differential media.
Gene expression may be controlled by insertion within an ________ unit
The _________ inactivates the promotor and does not allow attachment of _____ polymerase
An ________ exposes the promotor by having the molecule that was covering it to fall off
The process of creating mRNA from DNA is called _________
The process of reading mRNA to make proteins is called _________ and occurs at the _________
DNA directly taken from a eukaryotic organism is classified as __DNA (abbrev)
When a foreign gene is inserted into a plasmid it is now classified as __DNA (abbrev)
DNA made by the process of reverse transcription of mRNA is called __DNA as it now lacks introns
In order for bacteria to participate in conjugation it must possess the _______ factor and be ______.
If plasmids are transferred through a viral phage the process is called
_________ _______ are used to cut a plasmid vector for DNA insertion.
_________ is the most common type of bacteria used to make recombinant DNA.
The ______ site in a plasmid is the place where DNA polymerase attaches.
The _________ site in a plasmid is where RNA polymerase attaches and must be upstream from the inserted gene.
The ________ sequence signals the end of transcription in a plasmid.
Both ampr and bla are common __________ __________ genes inserted into engineered plasmids.
A _______ site is engineered into a plasmid to contain multiple restriction sites for gene insertion.
_______ is made by RNA polymerase
______ is the first codon in any gene sequence.
A 3 letter nucleotide sequence that describes an amino acid is a ________.
Genes that are required for normal bacterial function are called _________ genes.
Genes that are only turned on when needed are called __________ genes.
An ________ is a controlled unit of genes that are only turned on when needed and has been engineered into plasmids to control expression.
______ is a fluorescent selective marker that fluoresces only when turned on.
A bacteria that is able to receive pDNA is called ________.
The process of making cells membranes competent is called _________.
Once foreign DNA has been inserted into a plasmid it is regarded as ________ DNA.
The process of using foreign substances such as lipid vesicles to transfer pDNA is called _________.
________ plasmids are engineered plasmids that infect Eukaryotic cells.
Using a special gun designed to insert DNA coated pellets into cells is called the practice of
After sitting on ice the bacteria is _______ shocked at 50C to increase plasmid uptake.
The _____________ is a name given to the process of plasmid purification through liquid culture.
A ________ is a device that rapidly spins samples to separate its contents based on inertia.
The ___________ is the liquid above the pellet in a sample that has been spun in a centrifuge.
A _________ buffer is a substance that is used in the miniprep to cut the plasma membrane.
SDS/ alkaline/ lysis
A __________ is a device that quantifies bacteria in a sample based on light absorption.
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