Homework Problems

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Comparing sequences between genes and between species allows evaluation of the rates of change. Which of the following have been observed?
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Classify the gene below as either a tumor suppressor gene or an oncogene. BRCA1 is a(n)tumor suppressor geneWhich is often used as an insertional marker in a cloning vector?LacZa ddNTP is often used in the Sanger sequencing process. Compared to the normal DNA precursors, ddNTPs lack a(n) _____ at the ____ carbonOH; 3'One strand of double-stranded DNA is shown below. Assume that the other strand is also present. Is this sequence a palindrome? 5'-CATATG-3'yesThe process of separating DNA fragments based on their migration in an electrical current is calledelectrophoresisDNA is transferred from a gel to a nylon and hybridized to a DNA pole in which blotsouthernOne strand of double-stranded DNA is shown below. Assume that the other strand is also present. Is this sequence a palindrome? 5' - ATCGATGC - 3'noDuring gel electrophoresis, small pieces of DNA movefasterThe part of a bacterial cloning vector that contains many restriction enzyme recognition sequences is called amultiple cloning siteOne strand of double-stranded DNA is shown below. Assume that the other strand is also present. Is this sequence a palindrome? 5' - ACGCGT - 3'yes