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MLT 204 practical

Terms in this set (26)

ABO discrepancy
forward and reverse ABO grouping do not agree
focus on the low grade
ABO discrepancy affecting the ABO red cell testing
- extra antigen present:
+ B(A) phenotype/ test red cell with monoclonal antibody anti A reagent from another manufactures or a source of human polyclonal anti A
+ rouleaux: wash cell with saline
- missing or weak antigen: ABO weak or subgroup/ test with anti A1
- mix-field reaction: check transfusion history
ABO diiscrepancy affecting ABO serum or plasma testing
-extra antibodies: A subgroup with anti-A1/ test p.t red cell with anti A1 to see if subgroup A is present
- missing or weak antibody: newborn or elderly/ leave on the bench, incubate at 4C with autocontrol
ABO compatibility
AB universal plasma donor
O- universal donor of RBC
ABO phenotype rection
body titer antibody
- to determine an increase in strength during pregnancy
- 1+ reaction is end point
- an increase in the titer of more than 2 tubes is consider significant
p.t have clinically significant blood need
- perform full (AHG) cross match
- need blood antigen neg
when we do DC?- should neg result if not invalid
- AB pos- run DC to rule out Pana-agglutination
- run with weak D - check if IAT working property
RBC after transfusion
one unit of transfused RBC should increase the hematocrit by 3% and hemoglobin by 1g/dl
Homozygous
An organism that has two identical alleles for a trait
M+N-
Heterozygous
An organism that has two different alleles for a trait
M+N+
Fisher-Race and wiener race
Gel grading
weak D testing
donation and newborn for D neg mother
DAT
detect antibodies coating red cell
performed when suspected HDFN
check cell
used to validate neg test
on addition to a negative AHG test, the igG sensitized red cells should reactive with AHG reagent and show agglutination. if not invalid
clinical significance antibody
ABO
Rh
kell
Duffy
Lub
P
Ss
show dosage
EeCc
duffy
kidd
MN
antigen destroy
MNSs Duffy
antigen enhanced
Rh, Kidd, Lewis, I and P
high incidence antigen
anti-k, anti-Kpb, anti-Jsb, anti-Lub
Pano
- ruling out pos cell have neg AHG ( not those show dosage)
- matching pattern
- rule of 3
- circle those left
+ low incidence, don't need to worry unless crossmatch incompatible
+ high incidence, selective cell to rule out
- patient's phenotype
- give blood neg for that antigen
Cold reactive
IgM antibodies, such as anti-Lea, anti-Leb, anti-M, anti-N, anti-I, and anti-P1
AHG reactive
igG. Usually Fya, Jka, K,S
Low incidence antigens
V, Cw, Kpa, Jsa, Lua
React at both 37 and AHG
anti-E, anti-D, anti-c
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