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Micro prelab 1
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Terms in this set (12)
Wet mount slides are best viewed under 100 X magnification with immersion oil.
False
Which are the following rules about safety is correct?
A: Open toed shoes are fine to wear in the lab as long as you're wearing socks.
B: Very baggy shirts and pants are they preferred attire while working in the lab.
C: Wash your hands before you begin working, before you leave for the day, and anytime your hands or bench top potentially become contaminated with micro organisms.
D: You only have to treat microorganism safely if they called a really bad disease.
C
Total magnification
Ocular magnification X objective magnification
Resolution
The ability to see objects clearly enough to tell two distinct objects apart.
Magnification
Refers to the process of making an object appear larger than it is.
Low power objectives have a (large or small) field of view. That is why it is easier to locate your specimen on lower power objectives.
Large
If a microscope has an ocular magnification of 10x and an objective magnification of 100X, what is the total magnification of the microscope?
1,000x
it important to always use immersion oil when viewing slides under which objective?
100x
Which of the following statements about laboratory equipment is incorrect?
A: Disposable glass slides must be disposed of in special sharps containers to prevent safety hazards.
B: Microscopes are cheap to buy, therefore it's easy to replace them if they break due to negligence.
C: It is important to turn off the gas line whenever the flame is out on a Bunsen burner.
D: When any living creature is spilled, the spill must immediately be cleaned with a paper towel and disinfectant.
B
As the magnification increases, both the field of view and depth of field (increase or decrease).
Decrease
You cannot eat or drink anything in the lab, except for water.
False
When you are focusing a new slide, on which objective lens would you start your focus?
4x
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We often cut a watermelon in half and put it into the freezer to cool it quickly. But usually we forget to check on it and end up having a watermelon with a frozen layer on the top. To avoid this potential problem a person wants to set the timer such that it will go off when the temperature of the exposed surface of the watermelon drops to $3^{\circ} \mathrm{C}.$ Consider a 25-cm-diameter spherical watermelon that is cut into two equal parts and put into a freezer at $-12^{\circ} \mathrm{C}.$ Initially, the entire watermelon is at a uniform temperature of $25^{\circ} \mathrm{C},$ and the heat transfer coefficient on the surfaces is $22 \mathrm{W} / \mathrm{m}^{2} \cdot \mathrm{K}.$ Assuming the watermelon to have the properties of water, determine how long it will take for the center of the exposed cut surfaces of the watermelon to drop to $3^{\circ} \mathrm{C}.$
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