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39 terms

DNA Biology and Technology

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adenine (A)
One of four nitrogen-containing bases in nucleotides composing the structure of DNA and RNA
anticodon
Three-base sequence in a transfer RNA molecule base that pairs with a complementary codon in mRNA.
biotechnology
Term that encompasses genetic engineering and other techniques that make use of natural biological systems to create a product or achieve a particular result desired by humans,
codon
Three-base sequence in messengar RNA that causes the insertion of a particular amino acid into a protein, or termination of translation.
complementary base pairing
hydrogen bonding between particular purines and pyrimidines in DNA
cytosine (C)
One of four nitrogen-containing bases in nucleotides composing the structure of DNA and RNA; pairs with guanine
DNA fingerprinting
The use of DNA fragment lengths resulting from restriction enzyme cleavage to identify particular individuals.
DNA ligase
enzyme that links DNA fragments.
DNA polymerase
During replication, an enzyme that joins the nucleotides complementary to a DNA template.
DNA replication
Synthesis of a new DNA double helix.
frameshift mutation
Alternation in a gene due to deletion of a base, so that reading "frame" is shifted; can result in a nonfunctional protein.
gene mutation
a change in the base sequence of a gene
genetic engineering
Alternation of genomes for medical or industrial purposes.
guanine (G)
One of four nitrogen-containing bases in nucleotides composing the structure of DNA and RNA; pairs with cytosine
messanger RNA (mRNA)
type of RNA formed from a DNA template and bearing coded information for the amino acid sequence of a polypeptide
mRNA transcript
Faithful copy of the sequence of bases in DNA,
mutagen
Agent, such as radiation or a chemical, that brings about a mutation in DNA.
point mutation
Alterantion in a gene due to a change in a single nucleotide; results of this mutation vary.
polymerase chain reaction (PCR)
Technique that uses the enzyme DNA polymerase to produce millions of copies of a particular piece of DNA.
polyribosome
String of ribosomes simultaneously translating of the same mRNA strand during protein synthesis.
promoter
In a operon, a sequence of DNA where RNA polymerase binds prior to transcription.
recombinant DNA (rDNA)
DNA that contains genes from more than one source.
restriction enzyme
Bacterical enzyme that stops viral reproduction by cleaving viral DNA.
ribosomal RNA (rRNA)
Type of RNA found in ribosomes that translate messenger RNAs to produce proteins.
RNA
Mucleic acid produced from covalent bonding of nucleotide monomers thaty contain the sugar ribose.
ribosome
RNA and protein in two subunits; site of protein sythesis in the cytoplasm.
RNA polymerase
During transcription, an enzyme that joins nucleotides complementary to a DNA template.
semiconservative
Duplication of DNA resulting in two double helix molecules, each having one parental and one new strand.
template
Parental strand of DNA that serves as a guide for the complementary daughter strand produced during DNA replication.
thymine (T)
One of four nitrogen-containing bases in nucleotides composing the structure of DNA; pairs with adenine
transcription
the process whereby the DNA sequence in a gene is copied into mRNA
transfer RNA (tRNA)
type of RNA molecule that transfers amino acids to ribosomes during protein synthesis
transgenic organism
Free-living organism in enviroment that has a foregin gene inserted into it.
translation
process by which a sequence of nucleic acids in RNA is used to direct the production of a chain of specific amino acids
translocation
the movement of a segment of DNA from one chromosome to another, which results in a change in the position of the segment; also the movement of soluble nutrients from one part of a plant to another
transposon
DNA sequence capable of randomly moving from one site to another in the genome
triplet code
during gene expression, each sequence of three nucleotide bases stands for a particular amino acid
uracil (U)
A pyrimidine base found in nucleotides of RNA.
vector
Piece of DNA that can have a foregin DNA attached to it.