How can we help?

You can also find more resources in our Help Center.

44 terms

Microbiology Lab Quiz

Micro Lab Quiz for weeks 1-4
STUDY
PLAY
Parfocal
ability of microscope to stay in focus form one objective to another
Total magnification
ocular x objective
resolution
ability of the microscope to distinguish b/w tow separate points
wet mount
slide preparation of liquid suspension covered with a coverslip
simple stain
staining that uses one stain
prokaryotic
bacteria
no true nucleus
smaller than eukaryote
less comples
unicellular
eukaryotic
protozoa, fungus, animals
nucleus
membrane bound organelles
unicellular or multicellular
What is the function of a condenser?
condenses light to form a solid beam that focuses on specimen
What is the fuction of the ocular lens?
magnifies 10 x
What is the function of the objective lens?
magnifies 4x, 10x, 40x or 100x
What is the function of the diaphragm?
controls light to condenser
Is the protozoon euglena a prokaryote or eukaryote?
eukaryote
Is a bactium a prokaryote or eukaryote?
prokaryote
List 2 differences b/w a wet mount and a simple stain
-wet mount uses a slide cover and we view something alive
-simple stains use an oil and we kill everything with heat fix
aseptic technique
-procedure done to reduce contamination
-ex. flaming a loop, washing hands, keeping the lid on an agar plate
differential stain
-staining procedures that uses more than one stain
-ex. gram stain
streak plate
-method using sample of bacteria to be spread out over a surface
-goal: isolate colonies
Gram Stain Reactions
Positive:
Negitive:
Positive: purple
Negitive: pink
Bacterial Arrangements:
chains:
clusters:
pairs:
random:
chains: cocci or bacilli
clusters:cocci only
pairs: cocci only
random: bacilli only
Bacillus
Gram reaction:
Shape/ Morphology:
Arrangement:
Gram reaction: positive
Shape/ Morphology: rods
Arrangement: chain
E. coli
Gram reaction:
Shape/ Morphology:
Arrangement:
Gram reaction: negitive
Shape/ Morphology: rods
Arrangement: random
Staphylococcus
Gram reaction:
Shape/ Morphology:
Arrangement:
Gram reaction: positive
Shape/ Morphology: cocci
Arrangement: clusters
Complex agar
-non selective media that contains a variety of ingredientswith no set formula
-Base for most agars
-Contains nutrients to grow most bacteria
-Ex. Nutrient agar (NA), Tryptic Soy agar (TSA)
Enriched Agar
Complex agar enriched with other ingredients
Ex. BAP
Selective Agar
-Complex agar with an added ingredient to inhibit one type of organism while another type grows
-Ex. MAC (Selective for gram negative rods), MSA Selective for gpc (Staphylococcus species)
Differential Agar
-BAP can differentiate between bacteria that can hemolyze the red cells (break the red cells)
-Causes a clearing around the colony
TSA will grow:
gram positive & gram negitive bacteria
NA will grow:
gram positive & gram negitive bacteria
BAP will grow:
gram positive & gram negitive bacteria
MAC will grow
gram negitive bacteria
Lactose fermenters will grow:
pink colonies
MSA will grow
gram positive bacteria
Mannitol fermenters will grow:
yellow colonies
Metabolism
sum total of all reactions in the cell
Strict aerobe
organisms that require oxygen in order to grow.
Facultative anaerobe
organisms that use aerobic respiration when oxygen is present and fermentation when oxygen is absent. These organisms grow with and without oxygen.
Strict anaerobe
organisms that cannot grow in the presence of oxygen
Catabolism
process of breaking down large molecules into smaller units that can be used by the organism. Energy is released in the process.
Fermentation
metabolic reactions that convert energy contained in nutrients into ATP without oxygen
What environment is created when the mineral oil is added?
anaerobic
Is Pseudomonas an oxidizer and/or fermenter?
What type of bacteria is it?
oxidizer
strict aerobic
Is E. coli an oxidizer and/or fermenter?
What type of bacteria is it?
oxidizer and fermenter
facultative anaerobic
What is the jar called that produces an anaerobic environment?
Gas- Pack anaerobic jar
What type of bacteria can grow in an anaerobic environment?
strict anaerobe and facilitative anaerobe