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Light Source function

Provides radiant energy

Entrance Slit function

Provides a narrow beam of incoming light

Diffracting Grating function

allows single beam of light to be focused on one segment of visible spectrum

Exit Slit function

Focuses spectral segment on sample

Wavelength Selector

Moves the entrance slit across the colors of the spectrum provided by the Diffraction Grating

Sample Holder (Cuvette Holder)

Holds solution in Optical pathway


Converts radiant energy into electrical energy

Signal Processor

Modifies radiant energy to drive analog meter

Ways to calibrate a micropipette

using a balance to make sure that one ml is being delivered; 1mL=1g

Classes of water and their functions

Type 1: deionized water: results from ion exchange purification
Type 2: Double Distilled water: acceptable in most Clinical Labs; collected by boiling water and collecting the condensation
Type 3: used for washing dishes in lab

Ionic Strength

the combination of positive and negative charges of the ions in a solution

Colligative Properties

"Binding or fastening properties" examples: Vapor pressure, boiling point, freezing point, osmotic pressure


Have a single known concentration of a substance used to set up "Standard Curves"


Used to calibrate equipment and get measurements to fall in a certain range

RCF formulae

1.118 x 10^-5 x r x speed ^2

r= distance in cm from center to middle of 2nd row cuvette holder

How many Centimeters in an inch

2.54 cm

One point equation

Concentration (unknown)=[Absorbance (unknown)/Absorbance (standard)] x Concentration (standard)

Define Spectrophotometer

an instrument that measures monochromatic light

"0" adjuster knob function

used to compensate for background noise entering the cuvette well

What is measured in a spectrophotometer

Transmitted light

Beer's Law


A=absorbance a=molar absorptivity b=distance traveled by light c=concentration

Purpose of Standards Curve

to compare the absorbance of unknowns to the absorbance of standard solutions

Safety diamond

Red= flammability Blue= Health Hazards
Yellow= Reactivity White= Specific hazards (specials)

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