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Diffracting Grating function
allows single beam of light to be focused on one segment of visible spectrum
Moves the entrance slit across the colors of the spectrum provided by the Diffraction Grating
Ways to calibrate a micropipette
using a balance to make sure that one ml is being delivered; 1mL=1g
Classes of water and their functions
Type 1: deionized water: results from ion exchange purification
Type 2: Double Distilled water: acceptable in most Clinical Labs; collected by boiling water and collecting the condensation
Type 3: used for washing dishes in lab
"Binding or fastening properties" examples: Vapor pressure, boiling point, freezing point, osmotic pressure
1.118 x 10^-5 x r x speed ^2
r= distance in cm from center to middle of 2nd row cuvette holder
One point equation
Concentration (unknown)=[Absorbance (unknown)/Absorbance (standard)] x Concentration (standard)
Purpose of Standards Curve
to compare the absorbance of unknowns to the absorbance of standard solutions
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