Molecular biology - lab

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Plasmids are: (ch2)
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Terms in this set (25)
Plasmids are: (ch2)
DNA molecules separate from chromosomal DNA Self-replicating
Supernatant contains:(ch2)
• Plasmid DNA
• Soluble cellular constituents
Pellet contains: (ch2)
• PDS
• Lipids
• Proteins
• Chromosomal DNA
Why addition of ethanol on solution is important? (Ch3)
to pull DNA out of solution
Why we wash the DNA with 70% ethanol? (Ch3)
to remove residual CTAB
How we can handle with broken glassware in the lab? (Ch1)
Do not touch broken glassware with your hands.
Use a broom and dustpan to clean it up.
Dispose off broken glass in appropriate receptacles.
what must do Before leaving the lab? (Ch1)
-Return equipment and chemicals to their proper places
-Be sure to replace the lids to all containers
-Clean up your work area
Commonly used in molecular biology to determine the concentrations of DNA or RNA present in a mixture, as subsequent reactions or protocols using a nucleic acid sample often require particular amounts for optimum performance called.........(ch4)
Quantification of nucleic acids.
There are several methods to establish the concentration of a solution of nucleic acids, including : (Ch4)
• Spectrophotometric quantification.
• UV fluorescence in presence of a DNA dye.
Why spectrophotometers are commonly used to determine the concentration of DNA in a solution? (Ch4)
Because DNA and RNA absorb ultraviolet light, with an absorption peak at 260 nm wavelength.
What is the advantages of Organic Extraction of total RNA? (Ch5)Versatile Scalable Established and proven technology InexpensiveWhat is a disadvantages of Organic Extraction of total RNA? (Ch5)Organic solvents Not high-throughput RNA may contain contaminating genomic DNAThe DNA can be removed from the gel by....(ch6)Southern blotting.Why We use agarose gel electrophoresis?(ch6)to determine the presence and size of PCR products.Why we use paraffin oil in PCR reacted solution?(ch7-PCR Lab)used to avoid evaporation. when your amplification in the high temperature, the fluid will dry.Why we use bromophenol blue in PCR reacted solution?(ch7-PCR Lab)is coloring agent, marking the product.Why replication stops in the Sanger Technique?(ch8-DNA SEQUENCING Practical)Because they lack the -OH (which allows nucleotides to join a growing DNA strand).The Sanger Technique requires:(ch8-DNA SEQUENCING Practical) A- Multiple copies of single stranded template DNA. B- A suitable primer. C- DNA polymerase D- All of the aboveD- All of the aboveThe process of genetic alteration by pure DNA called.....(ch9-Bacterial Transformation)TransformationNumerate the steps of Bacterial Transformation:(ch9-Bacterial Transformation)1- Choose a plasmid to transform 2- Introducing plasmids into bacterial cells 3- Plate transformation solution on appropriate media 4- Incubate plates overnightE. coli grows in the human body and is therefore incubated at.....(ch9-Bacterial Transformation)body temperature ( 37 C )DNA can be extracted for analysis from........(ch10-SOUTHERN BLOTTING)hair, bones, saliva, sperm, skin, organs, all body tissues and blood.The deoxyribonucleic acid, DNA, is a long chain of nucleotides which consist of: (ch10-SOUTHERN BLOTTING) A-Deoxyribose(sugar with 5 carbons) B- Phosphate groups C- Organic(nitrogenous)bases D- All of the aboveD- All of the aboveIs a technique used for the study of gene expression called........(ch11&12-Southern, Northern and Western blotting)Northern Blotting.Northern Blotting is done by detection of:(ch11&12-Southern, Northern and Western blotting) A- particular RNA (or isolated mRNA). B- particular DNA. C- protein. D- ribosome.A- particular RNA (or isolated mRNA).