micro lab exam II

how do bacteria move? what is the purpose of motility?
Click the card to flip 👆
1 / 54
Terms in this set (54)
EMB contains __________, ___________, two _________, ___________, and _________ acid source, and the dyes __________ Y and ___________ bluelactose sucrose sugars peptone amino eosin methyleneon EMB, _______ lactose fermentation produces acids, which ________ the pH and stimulates dye absorption by the colonies that will develop a dark ________ color on the colonies.quick lowers purplein EMB, _______ lactose fermenters will develop a _________ _________ sheen on the surface of the colonies due to the metachromatic properties of the dyes in an _________ environmentavid metallic green acidicin EMB, organisms that are _______ lactose fermenters will use the _________ for growth and the deamination of amino acids can _________ the pH of the medium. colonies will appear ________ or ___________ on the plateslow peptone increase pink translucenton EMB, growth and color change can be seen after ______ hours, however it can be incubated for ________24 48the indole test is performed to determine the ability of the organism to produce ________ from the essential amino acid _____________indole tryptophanthe indole test used indole ________ and the presence of indole is detected by the addition of ________ drops of _________ reagentbroth 10 kovac'sa positive indole test is shown by the presence of a _______-_______ color on the __________. a negative test appears __________red violet surface yellowindole tests can be incubated for _____ hours48in the durham fermentation test (carbohydrates fermentation), acid production is identified by a change in the _______ of the media containing either _________ or _________, besides _______ extract and gelatin _________, and the pH indicator ______________ bluecolor glucose lactose beef peptone bromothymol(carbohydrates fermentation) in ________ conditions bromothymol blue changes to ________acid yellow(carbohydrates fermentation) to evaluate gas production, a small test tube is submerged _______ _______ in the culture tube to trap ________ ________ that might be _________ fermentationupside down gas bubbles releasedthe durham fermentation test can be incubated for _______ hours48simmon's citrate agar is used for the _________ _________ test and is a _________ medium that tests for an organism's ability to utilize ________ as a sole _______ source and ammonium ions as the sole _________ sourcecitrate utilization selective citrate carbon nitrogenthe citrate utilization test also contains the pH indicator ___________ _______ which is green at a pH below ______ then turns blue at a pH of _______ or greaterbromothymol blue 6.9 7.6(citrate utilization) the change in pH will occur with the ___________ of citrate, when carbon dioxide combines with ________ and _________ to form sodium bicarbonate, an ________ product that changes the color of the mediumdegradation sodium water alkalinevoges-proskauer test utilizes 2 reagents, A and B. A consists of ___% alpha-naphtol dissolved in _______ and B consists of ___% potassium hydroxide dissolved in _______5 ethanol 40 water(VP test) the reagents react with _______, oxidizing it to a _______ compound, forming a ______ complex that gives a ______ color to the mediaacetoin diacetyl pink rosethe VP test is used to __________ species of the ____________________ familydifferentiate enterobacteriaceae(VP test) after at least _____ hours, add ____ drops of reagent A and ____ drops of reagent B48 12 5urea can be utilized as a source of __________ by organisms that are able to ________ it, producing _____, water, and _________nitrogen hydrolyze CO2 ammoniathe urease test uses ______ _______ which contains _______ extract, two sources of __________, and the pH indicator ________ ________urea broth yeast phosphate phenol redurease activity will be observed by the change in _______. the phenol red will change from ________-________ to ________ as urea is _____________ and ammonia _____________, turning the medium __________color salmon orange fuchsia degraded accumulates alkalineurease test can be incubated for ________ hours48hemolytic activity is the ______ and destruction of _____ _______ ______lysis red blood cellsthe blood agar plate is _________ medium prepared with ___% citrated _______ blood which is rich in __________ and _______enriched 5 sheep proteins ironhemolytic activity test is useful to distinguish between the different types of gram________ cocci, mainly within the genre _______________, _______________, and _____________positive streptococcus enterococcus aerococcusalpha hemolysis is ___________ hemolysis caused by ____________ ___________ produced by the bacterium, oxidizing ____________ to green _________________. you will see a _________ zone of __-__ mm around the coloniesincomplete hydrogen peroxide hemoglobin methemoglobin green 1 3beta hemolysis is __________ lysis of the _______ blood cells in the media resulting in a ___________ to ___________ halo around the coloniescomplete red yellow transparentbeta hemolysis uses the enzyme ___________ that occurs in O and S types. O is an oxygen __________ cytotoxin and usually results in hemolysis _______ the ________ of blood agar. S is an oxygen _________ cytotoxin that will work on the ________ of the agarstreptolysin sensitive under surface stable surfacegamma hemolysis is _________ hemolysis. agar is ___________no unchangedwhat tests use gram negative bacteria?TSI, EMB, indole, durham fermentation, citrate, VP, ureasethe tellurite-glycine agar medium contains the carbohydrate ___________ besides ___________ tellurite and ___________ chloride and ___________ between S. ____________ and S. _______ speciesmannitol potassium lithium differentiates epidermidis aureusthe TGA test differentiates between _____________ positive (aureus) and and negative (epidermidis). organisms with the ability to ferment _________ and _________ tellurite will produce a precipitate that turns the colonies _________coagulase mannitol reduce blackincubate TGA tests for ______ hours24catalase is an __________ that breaks down ______ to produce water and ________ gas.exoenzyme H2O2 oxygenthe catalase test utilizes the ______ plate and a drop of ___% H2O2. the development of _________ indicates the __________ of the enzymeeugon 3 bubbles presencethe test used to identify DNase activity uses a medium that contains ________ and __________. DNA can be utilized as a source of __________ and ________ for growthDNA nutrients carbon energyDNase production may ________ virulence and help bacteria that produce the enzyme to evade _____________ actionincrease neutrophils(DNase test) add several drops of __________. if the plate develops a _________ appearance in the presence of HCL, then DNA is still present in the medium meaning ______ enzyme activity. if a ________ halo develops around the colonies, it means the enzyme ___________ _________1M HCL cloudy no clear hydrolyzed DNAbile esculin agar is used to __________ bile-resistant organisms based on their ability to _________ the compound __________ to __________ and __________differentiate hydrolyse esculin esculetin dextrose(bile esculin) esculetin reacts with _______ present in the medium to form a dark _______ or ________ precipitate that stains colonies and _________ mediumiron brown black surroundingbile is present as a _________ agentselectivewhat tests are used for gram positive bacteria?hemolytic, TGA, catalase, DNase, bile esculin