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5 Written questions

5 Matching questions

  1. ELISA is a example of immunoassay, indirect ELISA uses
  2. Zone of equivalence
  3. Hemagglutinin (HA), an antigenic glycoprotein from Influenza viruses morphology
  4. Agglutination reactions can occur with anti-lg-antibodies
  5. Immunoassays Multiple samples tested at once and has
  1. a particles with attached antigens+ complementary antibodies to antigens> antibodies bind to antigens but they can't cause agglutination> when anti-lg antibodies added, agglutination occurs
  2. b Great sensitivity much greater than other tests, dependent on type of tag
  3. c change the amount of antigen with constant amount of antibody or the other way around
  4. d -trimeric protein
    -13 major antigenic classes
    -functions in viral attachment to a variety of host cells
  5. e -antigen coated to plastic well
    -protein blocks remaining plastic surface
    -Serum added(primary antibody)
    -if antibodies(bind antigen)
    -if no antibodies(antigen not bound)
    -Indicator antibody(enzyme-linked anti-Ig antibody binds primary antibody)
    -Substrate(specific for enzyme linked to secondary antibody,enzyme causes substrate to change color)
    -Reactions(color change=
    antibody in serum,no color change=
    no antibody in serum)

5 Multiple choice questions

  1. -Similar to precipitation reaction
    -Visible reaction because antigen or antibody is on larger molecule (cell,latex bead,charcoal)
    -Interaction of antigen and antibody causes
    clumping of large particles
  2. rat hepatocytes b/c liver cells have large nuclei
  3. -using cells with antigen on them
    -naturally particulate antigens
  4. -patient sample on slide
    -ethanol to fix and kill
    -incubate with conjugated Ab ~25 min.
    -wash unbound Ab away
    -detect in fluorescent microscope
  5. -known Ag (ie, Toxoplasma gondii) on slide
    -incubate with patient serum 25 min.
    -wash unbound serum away
    -incubate with conjugated anti-Hu gamma globulin ~25 min.
    -wash unbound Ab away
    detect in fluorescent microscope

5 True/False questions

  1. In Gel precipitationred blood cells agglutinate
    certain viruses (influenza)


  2. Single immunodiffusion-Two antigens and one antibody and place in separate wells
    Allow diffusion
    -Lines of precipitation
    if continuous = identical antigens
    if crossing lines =completely different antigens
    if continuous with spur= partial identity


  3. Test issues (Specificity)eliminate false negatives


  4. Indirect Immunoassays isHormones (HCG, LH, HGH, FSH)
    Viral or bacterial antigens


  5. Types of precipitation reactions-using cells with antigen on them
    -naturally particulate antigens


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