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5 Written questions

5 Matching questions

  1. RapidStrep is VERY specific, but not
  2. Agglutination reactions can occur with anti-lg-antibodies
  3. In Double immunodiffusion
  4. Nurses performing more
  5. Immunoassays Detect antigen or antibody and use a secondary antibody tagged with
  1. a particles with attached antigens+ complementary antibodies to antigens> antibodies bind to antigens but they can't cause agglutination> when anti-lg antibodies added, agglutination occurs
  2. b POC testing than ever before because of the Immediate results
  3. c marker(radioactive,fluorescent,enzyme)
  4. d too sensitive, lots of false negatives because Monoclonal Ab based
  5. e -Two antigens and one antibody and place in separate wells
    Allow diffusion
    -Lines of precipitation
    if continuous = identical antigens
    if crossing lines =completely different antigens
    if continuous with spur= partial identity

5 Multiple choice questions

  1. eliminate false positives
  2. Screen for antibodies (anti-HIV, anti-rubella)
  3. -Ag is cardiolipin (beef liver)
    -specific test uses Ag from T. pallidum
  4. -indirect agglutination
    -measures IgE specific to a particular Ag
    -Ag-bead+pt serum IgE-Ag-bead+anti-IgE-125I
  5. Positive reaction:
    -Antibody present in serum
    -Serum added to test antigen
    -Bound antibody "fixes" complement
    -No available complement to lyse indicator cells
    Negative reaction
    -No antibody in serum
    -Complement not "fixed"
    -Free complement lyses indicator cells

5 True/False questions

  1. Monoclonal Antibody Production-Similar to precipitation reaction
    -Visible reaction because antigen or antibody is on larger molecule (cell,latex bead,charcoal)
    -Interaction of antigen and antibody causes
    clumping of large particles


  2. Visible soluble precipitate-results in mix soluble antigen and antibody
    -excess antigen or antibody--no precipitate
    -zone of equivalence--precipitate forms


  3. Immunofluorescence use Antibody with fluorescent label and theyGreat sensitivity much greater than other tests, dependent on type of tag


  4. Indirect Ab detection of Fluorescence microscopy procedure-patient sample on slide
    -ethanol to fix and kill
    -incubate with conjugated Ab ~25 min.
    -wash unbound Ab away
    -detect in fluorescent microscope


  5. Direct Agglutination reactions-using cells with antigen on them
    -naturally particulate antigens