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5 Written questions

5 Matching questions

  1. Qualitative agglutination
  2. Indirect Immunoassays is
  3. Indirect Agglutination reactions
  4. Visible soluble precipitate
  5. Agglutination reactions
  1. a -coated spheres or cells
    -observe agglutination
  2. b Screen for antibodies (anti-HIV, anti-rubella)
  3. c -results in mix soluble antigen and antibody
    -excess antigen or antibody--no precipitate
    -zone of equivalence--precipitate forms
  4. d -Similar to precipitation reaction
    -Visible reaction because antigen or antibody is on larger molecule (cell,latex bead,charcoal)
    -Interaction of antigen and antibody causes
    clumping of large particles
  5. e -Known antigen in fluid with Unknown specimen added
    -Agglutination =positive reaction
    -No agglutination=negative reaction
    -Similar to qualitative
    -Diluted samples of antibody
    -Measure amount of agglutination for each dilution

5 Multiple choice questions

  1. -Ag is cardiolipin (beef liver)
    -specific test uses Ag from T. pallidum
  2. -Bind to cell
    -Visualize under UV light
  3. POC testing than ever before because of the Immediate results
  4. eliminate false negatives
  5. -trimeric protein
    -13 major antigenic classes
    -functions in viral attachment to a variety of host cells

5 True/False questions

  1. Lupus - indirect test for anti-nuclear Ab (ANA) Usesrat hepatocytes b/c liver cells have large nuclei


  2. RAST - radioallergosorbent-indirect agglutination
    -measures IgE specific to a particular Ag
    -Ag-bead+pt serum IgE-Ag-bead+anti-IgE-125I


  3. Test issues (Specificity)eliminate false negatives


  4. Direct Ag detection of Fluorescence microscopy procedure-patient sample on slide
    -ethanol to fix and kill
    -incubate with conjugated Ab ~25 min.
    -wash unbound Ab away
    -detect in fluorescent microscope


  5. Agglutination reactions can occur with anti-lg-antibodiesparticles with attached antigens+ complementary antibodies to antigens> antibodies bind to antigens but they can't cause agglutination> when anti-lg antibodies added, agglutination occurs