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5 Written questions

5 Matching questions

  1. Indirect Immunoassays is
  2. In Gel precipitation
  3. Nurses performing more
  4. Complement fixation
  5. Zone of equivalence
  1. a Positive reaction:
    -Antibody present in serum
    -Serum added to test antigen
    -Bound antibody "fixes" complement
    -No available complement to lyse indicator cells
    Negative reaction
    -No antibody in serum
    -Complement not "fixed"
    -Free complement lyses indicator cells
  2. b change the amount of antigen with constant amount of antibody or the other way around
  3. c POC testing than ever before because of the Immediate results
  4. d -Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence
  5. e Screen for antibodies (anti-HIV, anti-rubella)

5 Multiple choice questions

  1. rat hepatocytes b/c liver cells have large nuclei
  2. -Immunization leads to Antibody-forming cells, which fuse with tumor cells to form hybridomas.
    -Hybidomas screened for antibody production and antibody-producing hybridomas cloned
    -Monoclonal antibodies isolated for cultivation
  3. marker(radioactive,fluorescent,enzyme)
  4. -trimeric protein
    -13 major antigenic classes
    -functions in viral attachment to a variety of host cells
  5. -known Ag (ie, Toxoplasma gondii) on slide
    -incubate with patient serum 25 min.
    -wash unbound serum away
    -incubate with conjugated anti-Hu gamma globulin ~25 min.
    -wash unbound Ab away
    detect in fluorescent microscope

5 True/False questions

  1. Hemagglutination-Known antigen in fluid with Unknown specimen added
    -Agglutination =positive reaction
    -No agglutination=negative reaction
    -Similar to qualitative
    -Diluted samples of antibody
    -Measure amount of agglutination for each dilution


  2. C. difficile test looks fortoxin, not bug


  3. Immunofluorescence use Antibody with fluorescent label and theyGreat sensitivity much greater than other tests, dependent on type of tag


  4. Direct Agglutination reactions-using cells with antigen on them
    -naturally particulate antigens


  5. Agglutination reactions can occur with anti-lg-antibodies-Similar to precipitation reaction
    -Visible reaction because antigen or antibody is on larger molecule (cell,latex bead,charcoal)
    -Interaction of antigen and antibody causes
    clumping of large particles