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5 Written questions

5 Matching questions

  1. In Gel precipitation
  2. Immunofluorescence use Antibody with fluorescent label and they
  3. Single immunodiffusion
  4. Agglutination reactions
  5. Indirect Immunoassays is
  1. a -Bind to cell
    -Visualize under UV light
  2. b -Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence
  3. c Screen for antibodies (anti-HIV, anti-rubella)
  4. d -Similar to precipitation reaction
    -Visible reaction because antigen or antibody is on larger molecule (cell,latex bead,charcoal)
    -Interaction of antigen and antibody causes
    clumping of large particles
  5. e -Antibody mixed into gel with specimens in well
    -screening for presence of antigen
    precipitate forms band around well which
    indicate presence of antigen
    -size of band relative to concentration of antigen

5 Multiple choice questions

  1. -antigen coated to plastic well
    -protein blocks remaining plastic surface
    -Serum added(primary antibody)
    -if antibodies(bind antigen)
    -if no antibodies(antigen not bound)
    -Indicator antibody(enzyme-linked anti-Ig antibody binds primary antibody)
    -Substrate(specific for enzyme linked to secondary antibody,enzyme causes substrate to change color)
    -Reactions(color change=
    antibody in serum,no color change=
    no antibody in serum)
  2. Test tube
    Capillary tube
    Agarose plate
  3. -trimeric protein
    -13 major antigenic classes
    -functions in viral attachment to a variety of host cells
  4. -Known antigen in fluid with Unknown specimen added
    -Agglutination =positive reaction
    -No agglutination=negative reaction
    -Similar to qualitative
    -Diluted samples of antibody
    -Measure amount of agglutination for each dilution
  5. too sensitive, lots of false negatives because Monoclonal Ab based

5 True/False questions

  1. Immunoassays Multiple samples tested at once and hasGreat sensitivity much greater than other tests, dependent on type of tag

          

  2. Test issues (Sensitivity)eliminate false positives

          

  3. Visible soluble precipitate-Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence

          

  4. Hemagglutinationred blood cells agglutinate
    certain viruses (influenza)

          

  5. Immunoassays Detect antigen or antibody and use a secondary antibody tagged with-Bind to cell
    -Visualize under UV light