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5 Written questions

5 Matching questions

  1. Direct Ag detection of Fluorescence microscopy procedure
  2. Agglutination reactions
  3. Visible soluble precipitate
  4. Qualitative agglutination
  5. Agglutination reactions can occur with anti-lg-antibodies
  1. a -Known antigen in fluid with Unknown specimen added
    -Agglutination =positive reaction
    -No agglutination=negative reaction
    -Similar to qualitative
    -Diluted samples of antibody
    -Measure amount of agglutination for each dilution
  2. b -patient sample on slide
    -ethanol to fix and kill
    -incubate with conjugated Ab ~25 min.
    -wash unbound Ab away
    -detect in fluorescent microscope
  3. c -results in mix soluble antigen and antibody
    -excess antigen or antibody--no precipitate
    -zone of equivalence--precipitate forms
  4. d -Similar to precipitation reaction
    -Visible reaction because antigen or antibody is on larger molecule (cell,latex bead,charcoal)
    -Interaction of antigen and antibody causes
    clumping of large particles
  5. e particles with attached antigens+ complementary antibodies to antigens> antibodies bind to antigens but they can't cause agglutination> when anti-lg antibodies added, agglutination occurs

5 Multiple choice questions

  1. -Antibody mixed into gel with specimens in well
    -screening for presence of antigen
    precipitate forms band around well which
    indicate presence of antigen
    -size of band relative to concentration of antigen
  2. marker(radioactive,fluorescent,enzyme)
  3. rat hepatocytes b/c liver cells have large nuclei
  4. Positive reaction:
    -Antibody present in serum
    -Serum added to test antigen
    -Bound antibody "fixes" complement
    -No available complement to lyse indicator cells
    Negative reaction
    -No antibody in serum
    -Complement not "fixed"
    -Free complement lyses indicator cells
  5. -using cells with antigen on them
    -naturally particulate antigens

5 True/False questions

  1. Nurses performing more-Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence

          

  2. Zone of equivalencered blood cells agglutinate
    certain viruses (influenza)

          

  3. Hemagglutinin (HA), an antigenic glycoprotein from Influenza viruses morphologyparticles with attached antigens+ complementary antibodies to antigens> antibodies bind to antigens but they can't cause agglutination> when anti-lg antibodies added, agglutination occurs

          

  4. The purpose of Immunofluorescence is to-Faster!
    -More field- and POC-friendly
    -Built-in controls for better accuracy/interpretation
    -Entamoeba histolytica

          

  5. Advantages of immunodiagnostics-Antibody mixed into gel with specimens in well
    -screening for presence of antigen
    precipitate forms band around well which
    indicate presence of antigen
    -size of band relative to concentration of antigen

          

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