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5 Written questions

5 Matching questions

  1. C. difficile test looks for
  2. Hemagglutinin (HA), an antigenic glycoprotein from Influenza viruses morphology
  3. Lupus - indirect test for anti-nuclear Ab (ANA) Uses
  4. Single immunodiffusion
  5. The purpose of Immunofluorescence is to
  1. a -detect specific proteins in cells
    -detect viruses in cells
    -identify microbial cells
    -identify and sort cells
    -fluorescent activated cell sorter (FACS)
  2. b -Antibody mixed into gel with specimens in well
    -screening for presence of antigen
    precipitate forms band around well which
    indicate presence of antigen
    -size of band relative to concentration of antigen
  3. c toxin, not bug
  4. d rat hepatocytes b/c liver cells have large nuclei
  5. e -trimeric protein
    -13 major antigenic classes
    -functions in viral attachment to a variety of host cells

5 Multiple choice questions

  1. -Faster!
    -More field- and POC-friendly
    -Built-in controls for better accuracy/interpretation
    -Entamoeba histolytica
  2. Positive reaction:
    -Antibody present in serum
    -Serum added to test antigen
    -Bound antibody "fixes" complement
    -No available complement to lyse indicator cells
    Negative reaction
    -No antibody in serum
    -Complement not "fixed"
    -Free complement lyses indicator cells
  3. particles with attached antigens+ complementary antibodies to antigens> antibodies bind to antigens but they can't cause agglutination> when anti-lg antibodies added, agglutination occurs
  4. marker(radioactive,fluorescent,enzyme)
  5. -Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence

5 True/False questions

  1. Indirect Ab detection of Fluorescence microscopy procedure-known Ag (ie, Toxoplasma gondii) on slide
    -incubate with patient serum 25 min.
    -wash unbound serum away
    -incubate with conjugated anti-Hu gamma globulin ~25 min.
    -wash unbound Ab away
    detect in fluorescent microscope

          

  2. Indirect Agglutination reactions-using cells with antigen on them
    -naturally particulate antigens

          

  3. In Double immunodiffusion-Antibody mixed into gel with specimens in well
    -screening for presence of antigen
    precipitate forms band around well which
    indicate presence of antigen
    -size of band relative to concentration of antigen

          

  4. Indirect Immunoassays isScreen for antibodies (anti-HIV, anti-rubella)

          

  5. Direct Immunoassays areScreen for antibodies (anti-HIV, anti-rubella)