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5 Written questions

5 Matching questions

  1. In Gel precipitation
  2. Immunofluorescence use Antibody with fluorescent label and they
  3. Direct Agglutination reactions
  4. RPR - syphilis test (indirect, screening) many false positives
  5. Single immunodiffusion
  1. a -Bind to cell
    -Visualize under UV light
  2. b -using cells with antigen on them
    -naturally particulate antigens
  3. c -Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence
  4. d -Antibody mixed into gel with specimens in well
    -screening for presence of antigen
    precipitate forms band around well which
    indicate presence of antigen
    -size of band relative to concentration of antigen
  5. e -Ag is cardiolipin (beef liver)
    -specific test uses Ag from T. pallidum

5 Multiple choice questions

  1. Test tube
    Capillary tube
    Agarose plate
  2. -Faster!
    -More field- and POC-friendly
    -Built-in controls for better accuracy/interpretation
    -Entamoeba histolytica
  3. -patient sample on slide
    -ethanol to fix and kill
    -incubate with conjugated Ab ~25 min.
    -wash unbound Ab away
    -detect in fluorescent microscope
  4. -Immunization leads to Antibody-forming cells, which fuse with tumor cells to form hybridomas.
    -Hybidomas screened for antibody production and antibody-producing hybridomas cloned
    -Monoclonal antibodies isolated for cultivation
  5. too sensitive, lots of false negatives because Monoclonal Ab based

5 True/False questions

  1. Complement fixationred blood cells agglutinate
    certain viruses (influenza)

          

  2. Indirect Agglutination reactions-using cells with antigen on them
    -naturally particulate antigens

          

  3. Test issues (Specificity)eliminate false negatives

          

  4. The purpose of Immunofluorescence is to-detect specific proteins in cells
    -detect viruses in cells
    -identify microbial cells
    -identify and sort cells
    -fluorescent activated cell sorter (FACS)

          

  5. Indirect Ab detection of Fluorescence microscopy procedure-patient sample on slide
    -ethanol to fix and kill
    -incubate with conjugated Ab ~25 min.
    -wash unbound Ab away
    -detect in fluorescent microscope