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5 Written questions

5 Matching questions

  1. Qualitative agglutination
  2. The purpose of Immunofluorescence is to
  3. Types of precipitation reactions
  4. In Gel precipitation
  5. Indirect Immunoassays is
  1. a Test tube
    Capillary tube
    Agarose plate
  2. b -detect specific proteins in cells
    -detect viruses in cells
    -identify microbial cells
    -identify and sort cells
    -fluorescent activated cell sorter (FACS)
  3. c -Known antigen in fluid with Unknown specimen added
    -Agglutination =positive reaction
    -No agglutination=negative reaction
    -Similar to qualitative
    -Diluted samples of antibody
    -Measure amount of agglutination for each dilution
  4. d -Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence
  5. e Screen for antibodies (anti-HIV, anti-rubella)

5 Multiple choice questions

  1. -indirect agglutination
    -measures IgE specific to a particular Ag
    -Ag-bead+pt serum IgE-Ag-bead+anti-IgE-125I
  2. Hormones (HCG, LH, HGH, FSH)
    Viral or bacterial antigens
  3. -Immunization leads to Antibody-forming cells, which fuse with tumor cells to form hybridomas.
    -Hybidomas screened for antibody production and antibody-producing hybridomas cloned
    -Monoclonal antibodies isolated for cultivation
  4. toxin, not bug
  5. particles with attached antigens+ complementary antibodies to antigens> antibodies bind to antigens but they can't cause agglutination> when anti-lg antibodies added, agglutination occurs

5 True/False questions

  1. ELISA is a example of immunoassay, indirect ELISA uses-antigen coated to plastic well
    -protein blocks remaining plastic surface
    -Serum added(primary antibody)
    -if antibodies(bind antigen)
    -if no antibodies(antigen not bound)
    -Indicator antibody(enzyme-linked anti-Ig antibody binds primary antibody)
    -Substrate(specific for enzyme linked to secondary antibody,enzyme causes substrate to change color)
    -Reactions(color change=
    antibody in serum,no color change=
    no antibody in serum)


  2. Zone of equivalencechange the amount of antigen with constant amount of antibody or the other way around


  3. In Double immunodiffusion-Two antigens and one antibody and place in separate wells
    Allow diffusion
    -Lines of precipitation
    if continuous = identical antigens
    if crossing lines =completely different antigens
    if continuous with spur= partial identity


  4. Single immunodiffusion-Antibody mixed into gel with specimens in well
    -screening for presence of antigen
    precipitate forms band around well which
    indicate presence of antigen
    -size of band relative to concentration of antigen


  5. Direct Agglutination reactions-using cells with antigen on them
    -naturally particulate antigens