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Clin Chem M3 Quiz

Terms in this set (20)

The component of an immunoglobulin molecule that determines the antigenic specificity of that antibody is the
sequence at the amino terminal end of the heavy and light chains.
Using whole blood as the specimen of choice in an automated analytical system
essentially eliminates specimen preparation time.
A substance that cannot elicit an immune response alone but that must be conjugated to a carrier molecule to cause antibody production is referred to as a(n)
hapten.
In regard to mass spectrometry, what is a molecular ion?
It is the unfragmented ion of the original molecule being studied.
The type of automated analyzer in which the operator is able to use in-house reagents or reagents purchased from suppliers different from the analyzer's manufacturer is referred to as a(n)
open analyzer system.
The type of separation mechanism for chromatography that involves, as one example, use of immunologic principles is _____ chromatography.
affinity
In partition chromatography, separation is based on
the differences in the relative solubility of compounds between the stationary and mobile phases.
What is the function of the vacuum system in a mass spectrometer?
To keep ions from colliding during interactions with the magnetic or electric fields
In a chemiluminescent analyzer system
the excitation event that produces light is caused by a chemical reaction.
In clinical applications using gas chromatography, which one of the following column temperature techniques will cause the more volatile analytes to elute first and therefore produce the best separation of complex mixtures of analytes with a range of volatilities?
Temperature programmed operation
You are preparing placental tissue for separation and isolation of a specific enzyme. The enzyme has a negative charge, so you want to use chromatography to perform your task. Which one of the following chromatographic separation mechanisms would be most useful?
Ion-exchange
The most significant improvement in the quality of laboratory test results can be attributed to the ability of automation to
reduce error and variability caused by manual manipulation.
The substance that is typically injected into an animal to elicit an immune response and induce formation of an antibody is the
immunogen.
In a mass spectrometer, the ion with the highest abundance in the mass spectrum that is assigned a relative abundance of 100% is referred to as the
base peak.
In the thin-layer chromatography procedure for drug screening, the retention factor (Rf) value for a compound is given by the
ratio of distance moved by the unknown solute to distance moved by the solvent in the mobile phase.
An antibody that has very strong affinity to a single antigenic epitope is referred to as a(n) _____ antibody.
monoclonal
Because of the ability to identify and quantify proteins in a complex mixture, mass spectrometry combined with separation methods is an excellent analytical tool used specifically in the field of
proteomics.
Some analytes must be derivatized to increase their column retention or detectability. Derivatization means
?
A measure of peak separation in a chromatographic method that equals the difference in retention time for two components divided by the average of their peak widths is the definition of
resolution.
In a precipitin reaction, an excess of antigen over an antibody will lead to
all antibody sites bound by antigen with no formation of precipitate.
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