Final Bio 206 Lab (Exam Questions)

What should not be disposed of in biohazard bins?
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What is the length of this plasmid (total number of base pairs)? (Using Exam 2's map)4361 base pairsYou perform a restriction digest using StyI and EcoR V enzymes: How many DNA fragments do you expect to see? (Use map in Exam 2)1.182 x 10^3 base pairsYou perform a restriction digest using StyI and EcoR V enzymes: What is the size of the DNA fragment(s)? (Use map in Exam 2)3179 base pairsYou perform a restriction digest using StyI and EcoR V enzymes: Which DNA fragment will migrate the least distance on an agarose gel? (Use map in Exam 2)The supercoiled and smaller DNA fragments will move slower in the gel: So, the 3179 base pairs will move slower.You were given an initial cell suspension and performed a 10,000-fold dilution using a saline solution to dilute the sample. After mixing you plated 200 microliters of the dilution and counted 25 colonies the next day. What was the density of cells in the initial suspension?1.25 x 10^6 cells/mLWhat is the correct order of steps when preparing media and pouring agar plates with temperature-sensitive additives (such as X-Gal and ampicillin) as described in the lab manual?1. Place media in the autoclave 2. Allow media to cool 3. Pipet additives into media 4. Pour media into Petri dishesYou want to make 50mL of a 3% (w/v) solution of agar. How much agar do you weigh out?1.5 gramsAn absorbance reading of 0 on a spectrophotometer Is equivalent to a transmittance reading of what?100%25 mL of starch solution is mixed with 25mL of pH 7.0 buffer and placed at 37 degrees Celsius. A 1mL sample is later removed and placed in a cuvette with iodine and mixed. Then, the percent transmittance is measured at 560nm. What treatment to the original 50mL mixture would give the highest percent transmittance?Adding 1.0mL pancreatic amylase solution to the mixture and incubating for 25 minutes.You are doing a series of pancreatic amylase experiments and find that in your most recent trial the reaction rate has increased compared to earlier trails. Which of the below is most likely responsible for your observations?You increased the substrate concentration.Which of the following reaction mixtures would most likely give the lowest percent transmittance at 560nm using a spectrophotometer? Assume that you use the same 1mL volume in the cuvette, add the same amount of iodine, and that no enzyme has been added yet.45mL of starch + 5mL of bufferWhat affects the affinity of an enzyme for a substrate?hydrogen bonding van der Waals interactions shape and conformationWhat is true about the relationship between Km and Vmax?Km is the substrate concentration that yields a reaction rate that is half of Vmax.In your amylase enzyme experiments, you monitored activity using a spectrophotometer. The _____ in % transmittance indicated the amylase was catalyzing the conversion of _____ to _____.increase; starch; maltoseWhich of the graphs in Exam 3 from an amylase experiment utilize a single flask?IIHow can hermaphrodite C. elegans reproduce?self-fertilization (selfing) mating with a maleA hermaphrodite worm is homozygous for a dominant mutation in an autosomal gene. It is crossed to a wild-type male. What phenotype would you expect to see int he F1 progeny if no selfing occurs?Mutant hermaphrodites and mutant malesWhy did you pick L2-L3 hermaphrodite larvae for the mating cross?To reduce the number of embryos resulting from selfing.The mechanism of RNAi uses _____ to _____.RNA; target mRNAOn your control plate containing rol-6 mutant hermaphrodite (rol-6m/m), you expected to see ____ that were ____ in the F1 progeny.hermaphrodites; rollersIf a single hermaphrodite worm heterozygous for an autosomal recessive mutation were placed on a plate to produce progeny via selfing, what would you expect to see among the progeny?A 1:3 ratio of mutant to normal womrsIf you analyzed an F1 generation and saw a male worm, he would most likely to be the result of the following?Cross-fertilization by the mating of hermaphrodite with a maleYou are trying to determine if an autosomal mutation in the dumpy gene is dominant or recessive. You select an adult hermaphrodite that you know is homozygous for the mutation (it has a dumpy phenotype) and cross it to a male that is homozygous with a wild-type allele. Assuming that some selfing and some mating occurs between the pair, what will convince you that the mutation is dominant?The presence of dumpy males