12 terms

Phenylalanine Deaminase Test (Micro)

STUDY
PLAY

Terms in this set (...)

Phenylalanine
A Amino Acid building block used when bacteria make protein, some can use it for energy.
What is the point of the Phenylalanine test?
To determine which bacteria can use Phenylalanine for energy.
What is Phenylalanine deaminase
It is the Enzyme which some bacteria use to convert Phenylalanine to Phenylpyruvic acid which bacteria then use for energy.
What is Deamination
It is a process by which a Amino group is removed. In this case Phenylalanine deaminase reduces Phenylalanine to Phenylpyruvic acid.
What kind of medium is used in the Phenylalanine deaminase test?
A Phenylalanine slant is used as the test medium. The medium is a regular Agar slant with extra Phenylalanine added. It has no indicators.
What is the indicator of the Phenylalanine deaminase test?
The indicator is Ferric Chloride (its a reagent)
What does a positive test result look like in the Phenylalanine deaminase test?
A positive result will cause the Ferric Chloride indicator to turn a green color, after it has been added to the inoculated and incubated slant.
What does a negative test result look like in the Phenylalanine deaminase test?
A negative test result will cause the Ferric Chloride to be yellow.
What does a positive test result mean in the Phenylalanine deaminase test?
It means that the bacteria has Phenylpyruvic acid present in it. Therefore the bacteria has the enzyme Phenylalanine deaminase, and said bacteria can use Phenylalanine for energy.
What does a negative test result mean in the Phenylalanine deaminase test?
It means that the bacteria does not have Phenylpyruvic acid present in it. Therefore the bacteria does not have the enzyme Phenylalanine deaminase, and said bacteria can not use Phenylalanine for energy.
How many drops of Ferric Chloride do you add to the inoculated Phenylalanine slant?
5 drops
What are the steps of the Phenylalanine test?
1. Inoculate phenylalanine agar slants with species 1:1
2. Incubate tubes at 37C till next class
3. Add 5 gtts of Ferric Chloride solution to the top of each slant, allowing the reagent to run through the growth on the slant.
4. Observe and record.