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Micro Lab Gram staining 🔬
terms, definitions, steps, order
Terms in this set (169)
Why do we use stains?
improve definition; obtain contrast between bacteria and background
What does a (-) negative stain show?
➖stains the, BACKGROUND of bacteria
What does a (+) positive stain show?
➕stains the BACTERIA itself
How are (+)positive stains classified?
as simple or differential; simple 👉 (1 stain used); differential 👉(2 or more stains used)
What information is provided by a simple stain?
basic information regarding⭕◼🔺 shape and arrangement of bacteria
What information is provided by a differential stain?
👊information about structure, or a part of the cell (cell wall);
✋whether parts are present (capsule/endospore);
👋 distinguishes between parts, identification of bacteria
Who invented Gram staining?
Christian Gram, standard now 1st test done to begin identifying🔬 bacteria
How does a gram stain differentiate between bacteria?
It differentiates between bacteria based on the structure of their CELL WALL
Gram negative cell wall
➖THIN layer of peptidoglycan;
surrounded by an outer membrane composed of phospolipids & lipopolysaccharides
Gram positive cell wall
➕THICK layer of peptidoglycan;
🚫no outer membrane
What color do Gram positive cells turn?
What color do Gram negative cells turn?
Why are Gram stains the most important in microbiology?
Because bacteria respond 1 of 2 ways;
1# it quickly identifies two major cell wall tpes; G (+) - thick peptidoglycan, purple G- thin peptidoglycan, pink
Why do bacteria respond differently to a Gram stain?
it responds differently because of the thickness of peptidoglycan in the cell walls; stains fix differently based on cell wall structure
What is the primary Gram stain?
Crystal violet stains all cells 🔮 purple regardless of cell wall structure
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