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70 terms

Micro Test 2

STUDY
PLAY
Which of the following is false regarding anaerobic respiration
It requires that 02 be present to occur
Which of the following statements is false about aerobic respiration
aerobic respiration produces a stable organic product
Fermentation differs from other forms of metabolism in that it
uses an organic substance as its final acceptor
which of the following is true about anaerocbic respiration
it uses part but not all of the krebs cycle
aerobic respiration differs from anaerobic respiration in which of the following aspects
the final electron acceptors are different
aerobic respiration requires an e transport chain
Aerobic respiration produces more atp
Which of the following may be an end product of fermentation
lactic acid
a bacteria that carries out only fermentation will produce enerdy
using glycolysis only
what is the false of pyruvate in an organism that carries out aerobic respiration
it's decarboxylates are oxidized to acetyl CoA
How many atp molecules are produced from aerobic respiration
38
how many atp molecules are produced from anaerobic respiration
3-37
metabolism is due
to the total chemical activity of an organism
Which of the following statements is true about an anabolic reaction
they build complex molecules from simple molecules
during chemiosmosis, ATP is produced because
protons are moved across the plasma membrane
The loss of one or more electrons from a substance is called
oxidation
The metabolic pathway, glycolysis, is used in which of the following metabolism
Fermentation
Anaerobic respiration
aerobic respiration
The most abundant product produced by the kreb cycle is
NADH
Which of the following is a physical growth requirement of bacteria
Temperature
Most common bacteria fall into which of the following t groups
mesophiles
most bacteria grow best a ph
7
selective media
inhibits the growth of certain bacteria
which of the following is a chemical or nutritional requirement for bacteria growth
o2
obligate anaeroces do not grow in the present of 02 because
oxygen is toxic to them
they lack the enzyme superoxide dismutase
o2 free radicals damage cellular components
a culture medium in which only G- bacteria can grow and some colonies are pink is called a
selective media
differential media
a culture medium in which the exact amount of each chemical found in the media is not known is called a
complex media
The term faculatative anaerobic refers to an organism that
grow in the presence or absence of energy
The phase of bacterial growth where the number of cells is constant and the cells are adapting to a new enviroment is called
lag phase
the enzyme that destroys superoxide free radicals in aerobic bacteria is called
superoxide dismutase
organic growth factors are
Substances that are required for bacterial growth
Substances that the organism can not synthesize on its own
such as vitamins
Where woychtrophs most likely be found
in spoiled food in the refridgerator
which of the following enzymes is used by abcteria to eleminate hydrogen peroxide
peroxidase
the basis of using turbidity as a measure of cell growth is
light scattering
All of the following are direct methods to measure microbial growth except
biomass
The method of direct measurement of microbial growth in which the microbes in a measurement volume of bacterial suspension are counted with the use of a specially designed slide is called
Direct microscope unit
a standard plate count
reflects the number of viable microbes in a samples
assumes that each bacterium grows into a single colony
requires that only plates with 25-250 colonies be counted
preservatives, such as sugar and salte, inhibit the growth of bacteria by creating a
hypertonic enviroment
Which of the following organism is able to neutralize toxic forms of 02 but grows using anaerobic respiration
aerotolerant anaerobic
which enzyme catalyze the breakdown of hydrogen peroxide to water and o2
catalase
which of the following substances found in culture medium is used to maintain a neutral ph
buffers
An obligate halophile will require high
salt
iodine is an example of a
halogen
dry-heat sterilization requires
temp of 170 c
ionization radiation is an effective method to control microbial growth by causing
the formation of free radicals
the phenol coefficient
involves testing 3 differnt bacterial stains for resistance
Compares the activity of a gram definfectant to the activity of phenol
A paper filter disk soaked in the agent to be tested
Moist heat is
Steam under pressure
Accomplished using an autoclave
The most efficient mean of controlling microbial growth
the mode of action of disenfectants is to affect
the cytoplasmic membrane
strucural proteins
essential proteins
which of the following is not a heavy metal
chlorine
commercial sterilization refers to a method of controlling microbial growth in which
enough heat is applied to kill the endospores of clostridium batulium
sterilization is a process by which
all forms of life in a given area are killed
which of the following affects the elimination of bacteria during sterilication
# of bacteria present
pH
Presense of organic matter
Surface active agents, such as soaps act by
sliding bacteria off the skin
which concentration of ethanol is the most effective bactericide
70%
non-ionizing radiation (UV) can kill bacterial cell by
causing thymine dimers to form m cellular DNA
which of the following is a possible target site for didinfectants
the nucear membrane
cell wall synthesis
enzyme synthesis
which of the following factors affect the elimination of microbes by chemical agents
-concentration
-exposure time
-temperature
the term bacteriocidal refers to a substance or method in which
bacteria are killed
The antimicrobial activity of chlorine is due to the formation of
Hypochlorous acid
Iodophors differ from iodine in that idophors are
Less irritating to host
Iodine bond to a neutral carrier molecule
Longer reacting
Which of the following would be a limitation of an autoclave
Using heat materials
The chemical agent used on skin or mucous membrane to eliminate microbes is called
an antiseptic
pasteurization is
involves heating milk to 70 c for 15 sec
Which of the following chemical agents can most reliably be used to sterilize objects
ethylene oxide gas
asceptically boxed juices and cream containers are processed using which of the following heating method
ultra high temp
a biguaride used extensively in antiseptic products is
chlorhexidine
a phenolic compound used in lotions and soaps that destroys cytoplasmic membranes and denatures proteins is called
triclosan
the temp and pressure combination for an autoclave is
121 c and 15 psi
ionizing radiation can be used for sterilizing
disposables
the best method to sterilized heat sensitive liquids is
filtration
which of the following are cold sterilants
peracetic acid and glutaraldehyde
which of the following is most resistant to destruction by chemicals and heat
endospores
which of the following does not achieve steriilization
pasteurization