Molecular Genetics Chapter 10
Terms in this set (16)
In E. coli, which terms accurately reflect the nature of replication of the chromosome?
bidirection and fixed point of initiation
DNA polymerase III adds nucleotides
to the 3' end of the RNA primer
Strucutres located at the ends of eukaryotic chromosomes are called
The discontinuous aspect of replication of DNA in vivo is caused by
5' to 3' polarity restriction
List at least four enzymes known to be involved in the replication of DNA in bacteria.
DNA Pol I and III, ligase, RNA primase, helicase, and gyrase
What structural circumstance in DNA sets up the requirement for its semidiscontinuous nature of replication?
5' to 3' polarity restrictions of DNA synthesis and the antiparallel orientation of the DNA strands in DNA
What protein is responsible for the initial step in unwinding the DNA helix during replication of the bacterial chromosome?
During DNA replication, what is the function of RNA primase?
RNA primase provides a free 3' OH upon which DNA polymerization depends
What primary ingredients, coupled with DNA polymerase I, are needed for the in vitro synthesis of DNA?
dNTP, DNA template, primer DNA or RNA, Mg2+ (appropriate buffering, temperature and salt concentrations might be considered "secondary" ingredients)
DNA replication in vivo requires a primer with a free 3' end. What molecular species provides this 3' end, and how is it provided?
The free 3' end is provided by an RNA primer, which is provided by the enzymatic activity of RNA primase
(T or F) DNA replicates conservatively, which means that one of the two daughter double helices is "old" and the other is "new."
False (double stranded parental strand is completely conserved)
(T or F) DNA strand replication begins with an RNA primer
(T or F) In the Meselson and Stahl (1958) experiment, bean plants (Vicia faba) were radioactively labeled so that autoradiographs could be made of chromosomes.
False ( used E. coli grown in N15 medium)
(T or F) A nucleosome is a structure associated with the nuclear membrane. It helps maintain a stable relationship between the extracellular matrix and the membrane itself.
False (form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it )
(T or F) A characterisitc of aging cells is that their telomeres become shorter
(T or F) Telomerase is an RNA-containing enzyme that adds telomeric DNA sequences onto the ends of linear chromosomes
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