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Microbiology Lab Midterm

Terms in this set (30)

What media did I use to select for fungal growth?
Potato Dextrose Agar
What part of the petri dish do you label?
Bottom
Which media did I use to swab areas for bacteria?
Plate Count Agar
What is the solidifying agent in solid media?
Agar
Microorganisms are ____________, so they are found everywhere.
ubiquitous
Our microscopes are ____________ meaning that they stay focused switching between objective lenses.
Parfocal
Our microscopes have __________ objective lenses.
4
What is the total magnification of the 4x objective lens?
40X
What is the resolving power of the 40X objective lens that has a numerical aperture of .55? Give the answer in µm. (Wavelength of visible light is 500nm)
0.454 µm
When focusing our microscopes, before the organism can be seen at the 100x objective lens, you must add _______________.
immersion oil
Which of the following is NOT a shape that bacteria come in?
Oval
What stain did we use in the simple stain that was performed in the video?
Crystal Violet
A _________ dye is used for the negative stain.
Anionic
What type of general dye is used in the simple stain?
Cationic
The crystal violet serves as the _____________ in the gram stain.
Primary stain
Gram positive cells have a _________ peptidoglycan layer which makes their cell wall composition different than gram negative organisms.
thick
The mordant in the gram stain is ________.
Iodine
Which cell does the primary dye rinse out of as soon as the decolorizer is used?
Gram negative
Motility agar has ______ agar which allows the bacteria to move.
0.5 %
A ___________ is an organism who can live or without oxygen.
Facultative Anaerobe
If a bacteria is moving in response to a light source this is called _________.
Phototaxis
What are the green objects inside of the pink cells?
Endospores
Before sporulation occurs the bacterial cell is known as the _______________ cell, this cell is actively metabolizing and reproducing.
vegetative
The primary dye in the schaeffer fulton spore stain is _________________.
Malachite green
To fix the primary dye into the spores _________ is applied to the primary dye.
Heat
Which of the following is an example of a spore forming bacteria?
Clostridium tetani
Which of the following is an acid fast organism?
Mycobacterium tuberculosis
When doing an acid fast stain what is the first dye we use?
Carbol Fuchsin
After the primary dye is applied in the acid fast stain, what is the smear rinsed with to decolorize?
Acid-alcohol
During the acid fast stain, after the decolorizing step the acid fast organisms are colorless.
False
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