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Microbio exam 2

Terms in this set (19)

Ocean Spray is very worried. Plastic bottles of its CranGrape juice are bulging and exploding on supermarket shelves across New England. Ocean Spray calls Dr. Strummer for help. Dr. Strummer samples CranGrape juice from a bulgin plastic bottle and isolates a species of Alicyclobacilli, an aerobic, acidophillic, mesophillic bacterium. He asks you, his faithful laboratory technician to culture the bacterium for future study.
You make a liquid media with a pH of _____ , inoculate the medium, and incubate the culture in a shaking incubator at _____.
3, 28oC
The tendency of a biological system to maintain internal stability, owing to the coordinated response of its parts to any situation or stimulus that would tend to disturb its normal condition or function is referred to as:
homeostasis
After testifying against his criminal conspirators, Tekashi69, real name Daniel Hernandez, has just entered the FBI witness protection program. While working at his new day job in construction, Tekashi69 "falls" from a two story window, leaving him unable to walk and in incredible pain. His handler, Jack Webb, takes Tekashi69 to his local area hospital hoping that his prominent facial tattoos will not blow his cover. Physician's diagnose Tekashi69 with a shattered hip and say he is in need of an immediate hip joint replacement. Dr. Octagon, the consulting orthopedic surgeon, prepares for surgery but notices the packaging of the hip prosthetic he needs to use has been compromised and is no longer sterile. The prosthetic is composed of metal and plastic, stable at temperatures under 100oC, sensitive to ionizing radiation, and chemically non-reactive. Which sterilization technique would you recommend Dr. Octagon use to sterilize the hip prosthetic?
Formaldehyde Gas Vapor
An antiseptic is distinguished from other chemical disinfectants by:
its ability to be used on living tissues without harm.
Dr. Strummer is comparing three chemical disinfectants, Lysol, tincture of iodine, and chloramine, for their ability to kill Staphylococcus aureus. Lysol has a phenol coefficient of 3.5. Tincture of iodine has a phenol coefficient of 6.3. Chloramine has a phenol coefficient of 133. Which chemical agent is the least effective at killing Staphylococcus aureus?

Lysol
tincture of iodine
chloramine
Lysol
Sterilization indicates
the elimination of all microbes.
Soap combined with hot water is considered:
a low level disinfectant.
____________ is all of the biochemical reactions taking place in an organism.
Metabolism
In an oxidation reduction reaction, the substance reduced _____ electrons and the substance oxidized _____ electrons.
gains, loses
Cellular energy can be stored in the form of ATP
False
FAD+ is required for the Citric Acid Cycle.
true
Which of the following metabolic pathways generates ATP through oxidative phosphorylation?
Electron Transport System
Glycolysis converts glucose into two _____ carbon molecules of pyruvate, generating a net _____ ATP and _____ NADH.
3, 2, 2
Glycolysis and the Citric Acid cycle generate ATP via:
substrate-level phosphorylation
The Proton Motive Force or Δp, generated by the pumping of protons from the cytoplasm to the periplasmic space creates:
both an electrical potential (Δψ) and pH difference (ΔpH)
Electrons are transferred between proteins in the electron transport chain using:
Quinones
In aerobic respiration, the final electron acceptor is:
O2
True or False: Dr. Strummer can test a new strain of Alicyclobacillus acidocaldarius, a thermophile, to determine its thermal death time at 60oC.
False
UV irradiation works as a disinfectant procedure because:
it damages bacterial DNA by causing the formation of Thymine dimers.
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