1.Microorganisms exist everywhere except on sterilized equipment, 2. Frequent handwashing and maintaining intact skin are the best methods for reducing the transmission of microorganisms, 3. Blood, body fluids, cells, and tissues are considered major reservoirs of microorganisms, 4. Personal protective equipment such as gloves, gowns, masks, goggles, and hair and shoe covers serves as a barrier to microbial transmission, 5. A clean enviornment reduces microorganisms, 6. Certain areas-the floor, toilets, and inside of sinks-are more contaminated than others. Cleaning should be done from cleaner to dirtier areas.