Eye levelHow can we read a volumetric measurement to avoid erroneous measurements due to parallax error?Decrease as it is heated, increase when coldHow will temperature affect volume measurement of fluid?Accurate and precise pipettingThey are skills required to prepare various diluents such as reagents and px sample when performing quantitative measurements in the clinical laboratoryPipettesA volumetric glassware that is used extensively in the laboratory20 degcelAt what temperature pipettes are designed to accurately deliver fluid?Volumetric (transfer), Graduated (measuring)2 Types of Manual PipetteVertical positionPipette must be held at what position?Contraction and expansionWhat factors can affect when fluids are not measured at room temperature?Soak in 5% HCl / 5% nitric acidHow can we pre-treat a new and unused pipette?Water + CuvetteComposition of Blank TubeSample + CuvetteComposition of Sample BlankReagent + CuvetteComposition of Reagent Blank•Pipette to be used must be the closest to the volume needed•Dirt and ships may alter volume•Do not wipe the tip, it could flick away liquid inside•Hold with three fingers and thumb, index finger must be free•Volumetric pipette is not a blown-out pipette, to remove water the tip must touch inside of receiving vessel•Serologic pipette must be blown out to drain fluid•Fluid expands when heated and contracts when cold•Bubbles invalidates the volume of liquid to be delivered. Resample.•Dropping a pipette alters calibration•Pre-wet pipette to flush out residual substances
