Activity 5: Glucose Oxidase

Glucose
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Terms in this set (20)
Glucose
It is a major carbohydrate present in the peripheral blood
Oxidase of glucose
It is the major source of cellular energy in the body
Diagnosis and treatment of diabetes mellitus
Glucose determination are primarily for?
Pancreatic, Pituitary, Thyroid dysfunction, Renal failure, Liver disease
Elevated glucose levels are associated with what diseases? (5-PPTRL)
Insulinoma, Hypopituitarism, Neoplasm, Insulin-induced hypoglycemia
Low glucose levels are associated with what diseases? (3-IHNI)
Human serum
Specimen for Glucose oxidase method?
7% per hour
In whole blood, at what percentage does glucose decreases if not separated from serum from the clot?
24 hours
How long is glucose in serum stable at 2-8degcel?
2-8 degcel
At what temp is glucose in serum stable?
50mL distilled water
Glucose reagent is reconstituted with?
Glucose Oxidase, Horseradish peroxidase, Mutarotase, 4-aminoantipyrine, P-hydroxybenzene sulfonateComposition of Glucose reagent (5-GHM4P)2-8degcel in amber containerProper storage of glucose reagent to remain stability30 daysOpened/Reconstituted glucose reagent is stable for how long?Reagent BlankWhat must be used to zero the spectro?Distilled/deionized waterWhat is used for reconstitution?Gross hemolysis, lipemia, icterus, anticoagulantsInterfering substances in glucose oxidase method (4-GLIA)Citrate, EDTA, heparinAnticoagulants that are interfering substances in glucose oxidase method (3-CEH)Serum blankWhat is used to correct gross hemolysis, lipemia and icterus?20 uL of serum to 3 mL distilled waterComposition of serum blank500 mg/dLAnalytical measurement range