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Terms in this set (166)
Which is used to calibrate the refractometer?
Refractometer standard
Normal saline
Distilled water
Control serumDistilled waterWhat designation is used to describe a pipette that has a double-etched or frosted band at the top?
"To contain with rinsing"
"To deliver with blow-out"
"To contain"
"To deliver""To deliver with blow-out"Which type of pipette is used only to add liquid to another liquid and must then be rinsed with the first liquid?
Transfer
Volumetric
"To contain"
"To deliver with blow-out""To contain"What is used to clean the optical surface of the refractometer immediately after use?
Xylene
Soap and water
Kimwipes
Lens paperLens paperWhat type of tube is used to prepare a urine sample prior to centrifugation?
Sterile
Conical
Microhematocrit
Blood collectionConicalWhat item is required for the processing of blood components?
Refrigerated centrifuge
Standard clinical centrifuge
Flat-head centrifuge
Angled-head centrifugeRefrigerated centrifugeWhich of the following does not require an adapter for use with different sizes of test tubes?
Simple standard water baths
Heat blocks
Circulating water baths
Waterless bead bathsWaterless bead bathsWhich term is used to describe flat field objective lenses?
Planachromatic
High-eyepoint
Abbe
ApochromaticPlanachromaticWhich component of the microscope functions to aim and focus the light that is illuminating the specimen?
Nosepiece
Iris diaphragm
Aperture diaphragm
CondenserCondenserWhich component of the microscope regulates the amount of light that is illuminating the specimen?
Condenser
Nosepiece
Iris diaphragm
Coarse adjustment knobIris diaphragmWhat is the preferred cleaning solution to be used for the routine cleaning of microscope lenses?
Methanol
Ammonia
Xylene
Mineral oilMethanolWhat is the preferred cleaning solution to be used for the removal of excess oil from the microscope lenses?
Mineral oil
Xylene
Ammonia
MethanolXyleneWhich measurement is equal to 1 kg?
1000 g
100 mg
100 g
1000 mg1000 gWhat is the final dilution of a solution that contains 20 mg/dL of a substance that is diluted 1 : 20?
20 mg/dL
2 mg/dL
10 mg/dL
1 mg/dL1 mg/dLWhich term refers to how close a test result is to the actual patient value?
Accuracy
Sensitivity
Specificity
PrecisionAccuracyWhich term refers to the reproducibility of a test result?
Sensitivity
Specificity
Accuracy
PrecisionPrecisionWhich term describes a product that is analyzed in the same manner as a patient sample and used to verify test results?
Reagent
Control
Calibrator
StandardControlWhich term describes the ability of a testing method to be accurate and precise?
Precision
Reliability
Accuracy
ReproducibilityReliabilityCollecting a blood sample from a patient that is not properly fasted is an example of which type of error?
Nonbiologic
Postanalytic
Analytic
PreanalyticPreanalyticAn improperly maintained analyzer can introduce which kind of error into a test result?
Analytic
Nonbiologic
Postanalytic
PreanalyticAnalyticHemoglobin formation begins during the __________ stage of erythrocyte maturation and ends during the __________ stage.
Rubricyte; reticulocyte
Rubriblast; reticulocyte
Metarubricyte; reticulocyte
Rubricyte; metarubricyteRubricyte; metarubricyteWhat is the primary cytokine involved in the stimulation of erythrocyte production?
Leukopoietin
Erythropoietin
Thrombopoietin
HematopoietinErythropoietinWhat is the primary cytokine involved in the stimulation of platelet production?
Thrombopoietin
Erythropoietin
Leukopoietin
HematopoietinThrombopoietinWhat is the primary cytokine involved in the stimulation of leukocyte production?
Thrombopoietin
Erythropoietin
Hematopoietin
LeukopoietinLeukopoietinThe cells in the erythrocyte maturation series in order from most immature to most mature are:
Proreticulocytes, metarubricytes, rubricytes, reticulocytes
Rubricytes, rubriblasts, proreticulocytes, reticulocytes
Rubriblasts, prorubricytes, metarubricytes, rubricytes, reticulocytes
Rubriblasts, prorubricytes, rubricytes, metarubricytes, reticulocytesRubriblasts, prorubricytes, rubricytes, metarubricytes, reticulocytesThe cells in the granulocyte maturation series in order from most immature to most mature are:
Myeloblast, myelocyte, promyelocyte, metamyelocyte, band cell
Myeloblast, promyelocyte, metamyelocyte, myelocyte, band cell
Promyeloblast, myeloblast, myelocyte, metamyelocyte, band cell
Myeloblast, promyelocyte, myelocyte, metamyelocyte, band cellMyeloblast, promyelocyte, myelocyte, metamyelocyte, band cellAfter a granulocyte matures to the __________ stage, it begins to exhibit granules that are characteristic of the neutrophil, the eosinophil, or the basophil.
Correct! Myelocyte
Myeloblast
Metamyelocyte
PromyelocyteWhich cells are part of the granulocyte proliferation pool?
Promyelocytes and metamyelocytes
Metamyelocytes and band cells
Correct! Myeloblasts, promyelocytes, and myelocytes
Myeloblasts and metamyelocytesWhich term describes a decrease in the numbers of all blood cells and platelets?
Reticulocytosis
Polycythemia
Erythropenia
Correct! PancytopeniaWhat is meant when a sample is described as having a left shift?
A leukemoid response
Correct! An increase in the number of immature neutrophils in the blood
A shift in the neutrophil-lymphocyte ratio in favor of neutrophils
NeutrophiliaThe Vacutainer that is most suitable for collection of blood for hematology usually has a __________ colored top.
Green
Correct! Purple or lavender
Red
BlueWhich anticoagulant is preferred for routine hematologic studies because it preserves cell morphology?
Correct! EDTA
Sodium citrate
Ammonium oxalate
HeparinWhich anticoagulant provides the best preservation of glucose?
Correct! Sodium fluoride
Heparin
EDTA
Ammonium oxalateWhat can occur if a tourniquet is left in place for an excessive amount of time?
Hemolysis
Icterus
Lipemia
Correct! HemoconcentrationWhen collecting samples from a patient who requires hematology, chemistry, and coagulation testing, the first sample collected will be the one that requires __________ anticoagulant.
Fluoride
EDTA
Correct! Citrate
NoWhen collecting samples from a patient who requires hematology and chemistry testing, the first sample collected will be the one that requires __________ anticoagulant.
EDTA
Fluoride
Correct! No
CitrateWhat is contained in the "tiger-top" blood collection tube?
Oxalate
EDTA
Silicone
Correct! Gel separatorWhich type of analyzer counts particles on the basis of their size?
Quantitative buffy coat analyzer
Refractometer
Correct! Impedance counter
Laser flow cytometerWhich type of instrument counts particles on the basis of their relative size and density?
Quantitative buffy coat analyzer
Impedance counter
Correct! Laser flow cytometer
RefractometerWhich instrument provides an estimate of cell counts on the basis of differential centrifugation?
Impedance counter
Correct! Quantitative buffy coat analyzer
Laser flow cytometer
RefractometerRunning the electrolyte solution through the impedance counter to identify the presence of small particles so that the analyzer does not count them is referred to as the:
Histogram output
Correct! Background count
Agglutinin zero point
Threshold control testSamples from __________ may not be adequately evaluated with impedance analyzers as a result of the size similarities between red blood cells and platelets.
Correct! Cats
Horses
Dogs
CowsWhat is the volume of each of the nine squares of the hemocytometer with the Neubauer ruling?
0.1 µL
0.4 µL
0.9 µL
1.1 µL0.1 µLWhich term describes an increase in the number of circulating erythrocytes?
Polycythemia
Pancytopenia
Erythropenia
ReticulocytosisPolycythemiaWhich of the following is a possible cause of relative polycythemia?
Excess EPO in the blood
Tumors
Splenic contraction
Lung diseaseSplenic contractionWhich of the following is a common cause of increased plasma protein levels?
Renal disease
Overhydration
Dehydration
Liver diseaseDehydrationWhich instrument is used to measure the total protein content of a sample?
HemoCue
Impedance counter
Centrifuge
RefractometerRefractometerThe normal packed cell volume in dogs is about:
32%
52%
45%
35%45%What is the MCV in a canine patient with a PCV of 38% and a RBC count of 6.5 × 106/µL?
5.8 fL
58 fL
1.7 pg
17 pg58 fLWhat is the unit of measure for MCHC?
%
fL
pg
g/dLg/dLAn estimate of the total red blood cell count can be obtained by dividing the __________ by 6.
MCHC
PCV
MCH
Hemoglobin levelPCVWhen making a blood smear with a sample that is very thick and that contains some small clots, you should do which of the following?
Increase the spreader slide angle to about 45 degrees.
Decrease the spreader slide angle to about 20 degrees.
Dilute the sample 2 : 1 with EDTA.
Make the smear from a fresh sample.Make the smear from a fresh sample.What is the predominant white blood cell type in ruminants?
Neutrophil
Monocyte
Eosinophil
LymphocyteLymphocyteWhich cell type is characterized by amoeboid nuclear material and abundant, blue-gray, vacuolated cytoplasm?
Neutrophil
Eosinophil
Monocyte
LymphocyteMonocyteWhich white blood cell is the predominant one that responds to allergies and parasitic disease?
Monocyte
Neutrophil
Eosinophil
LymphocyteEosinophilWhich cell type can be identified by its dark, irregular, lobulated nucleus and its colorless or pale-pink cytoplasm?
Neutrophil
Monocyte
Eosinophil
LymphocyteNeutrophilThe cell with a rounded or slightly indented nucleus that almost completely fills the cell is the mature __________.
Lymphocyte
Eosinophil
Neutrophil
MonocyteLymphocyteWhich of the following best describes the granules of feline eosinophils?
Rod-shaped
Small and round
Large and oval
Varying sizeRod-shapedWhich is the largest white blood cell in the peripheral blood?
Neutrophil
Monocyte
Lymphocyte
EosinophilMonocyteIf a patient has 70% neutrophils on the differential blood cell film and a white blood cell count of 12,000, what is the absolute number of neutrophils present per µL?
8400
20,400
840
24008400Phagocytosis is the primary function of which cell?
Lymphocyte
Eosinophil
Neutrophil
BasophilNeutrophilWhich of the following best describes the morphology of canine erythrocytes?
Biconcave disc
Round
Elliptical
NucleatedBiconcave discWhich of the following best describes the morphology of avian erythrocytes?
Round
Biconcave disc
Nucleated
EllipticalNucleatedA platelet estimate that is performed with the use of a differential blood cell film requires the counting of platelets in a minimum of _____ microscopic fields.
6
10
20
1510Multiply the platelet estimate obtained from the blood film by _____ to get the platelet estimate per µL of blood.
50,000
10,000
20,000
5,00020,000Which of the following can be seen in lead poisoning?
Basophilic stippling
Echinocytosis
Spherocytosis
AnisocytosisBasophilic stipplingWhich abnormality is characterized by grapelike clusters of red blood cells that do not break up when the sample is diluted with saline?
Heinz bodies
Rouleaux
Howell-Jolly bodies
AgglutinationAgglutinationWhich red blood cell abnormality is often seen in healthy horses?
Rouleaux
Howell-Jolly bodies
Heinz bodies
AgglutinationRouleauxWhich term describes pale or bluish round areas attached to the red blood cell membrane that are caused by chemical- or drug-induced oxidative injury?
Heinz bodies
Howell-Jolly bodies
Agglutination
RouleauxHeinz bodiesWhich abnormality is characterized by coinlike stacks of red blood cells?
Howell-Jolly bodies
Rouleaux
Agglutination
Heinz bodiesRouleauxWhich term describes round basophilic nuclear remnants in the red blood cells of animals with regenerative anemia?
Howell-Jolly bodies
Heinz bodies
Agglutination
RouleauxHowell-Jolly bodiesWhich of the following is a toxic change that may be observed in neutrophils?
Heinz bodies
Nuclear hyposegmentation
Döhle bodies
Howell-Jolly bodiesDöhle bodies. The presence of basophilic macrocytes on a peripheral blood cell film usually indicates increased numbers of:
Reticulocytes
Metarubricytes
Dacrocytes
CodocytesReticulocytesWhich term describes cells with regular cytoplasmic projections around the periphery that give the cell the appearance of a scalloped border?
Keratocytes
Acanthocytes
Echinocytes
SchistocytesEchinocytesSmall round or rod-shaped structures that are found as single organisms or pairs on the periphery of a red blood cell are:
Döhle bodies
Ehrlichia
Mycoplasma
Heinz bodiesMycoplasmaWhich type of stain is used to prepare a reticulocyte count smear?
Diff-Quik
Wright's
Supravital
RomanowskySupravitalHow is the reticulocyte count recorded if a reticulocyte smear from a cat has 5 punctate reticulocytes and 15 aggregate reticulocytes per 1000 red blood cells?
2.0%
2.5%
0.5%
1.5%1.5%How is the reticulocyte count recorded if a reticulocyte smear from a dog has 25 reticulocytes per 1000 red blood cells?
2.0%
0.5%
1.5%
2.5%2.5%. What is the absolute value of reticulocytes if 200 reticulocytes are counted per 1000 red blood cells and the total red blood cell count is 2.2 × 106/µL?
220,000
440,000
22,000
44,000440,000The presence of basophilic macrocytes on a peripheral blood cell film usually indicates increased numbers of:
Reticulocytes
Metarubricytes
Dacrocytes
CodocytesReticulocytesWhich term describes cells with regular cytoplasmic projections around the periphery that give the cell the appearance of a scalloped border?
Keratocytes
Acanthocytes
Echinocytes
SchistocytesEchinocytesSmall round or rod-shaped structures that are found as single organisms or pairs on the periphery of a red blood cell are:
Döhle bodies
Ehrlichia
Mycoplasma
Heinz bodiesMycoplasmaWhich type of stain is used to prepare a reticulocyte count smear?
Diff-Quik
Wright's
Supravital
RomanowskySupravitalWhat is the corrected reticulocyte percentage if a canine patient has 10% reticulocytes and a packed cell volume of 32%?
4.2%
8.6%
3.2%
7.1%7.1%What is the corrected reticulocyte percentage if a feline patient has 10% reticulocytes and a packed cell volume of 32%?
9.1%
7.1%
4.2%9.1%What is the minimum number of nucleated cells that are counted and classified to calculate the ratio of myeloid cells to erythroid cells in bone marrow?
500
200
1000
100500How would a bone marrow sample from an adult animal be described if it contains 80% fat?
Hypercellular
Normal
Aplastic
HypocellularHypocellularWhat stain is used to evaluate the presence of hemosiderin in a bone marrow sample?
Diff-Quik
Wright's
Prussian blue
Wright-GiemsaPrussian blueWhat is the common term that is used to describe the presence of neoplastic blood cells in the bone marrow and the peripheral blood?
Myeloproliferative
Lymphoproliferative
Leukemia
PyogranulomatousLeukemiaWhich of the following is likely to cause microcytic anemia?
Hypothyroidism
Ehrlichiosis
Drug toxicity
Iron deficiencyIron deficiencyWhich term describes chronic bone marrow inflammation that is characterized by increased numbers of macrophages?
Fibrinous
Hypoplastic
Pyogranulomatous
GranulomatousGranulomatousWhich term describes chronic bone marrow inflammation that is characterized by increased numbers of macrophages and neutrophils?
Hypoplastic
Pyogranulomatous
Fibrinous
GranulomatousPyogranulomatousThe classification of anemia according to __________ distinguishes between regenerative and nonregenerative anemia.
Bone marrow response
Erythrocyte indices
Morphology
EtiologyBone marrow responseWhat is the molecule that platelets express on their surface when they are activated?
Von Willebrand factor
Microparticles
Thrombin
PhosphatidylserinePhosphatidylserineWhich molecule binds to tissue factor in the plasma to initiate the coagulation reactions?
Factor VIII
Thrombin
Von Willebrand factor
ProthrombinFactor VIIIWhich of the following functions to stabilize the platelet plug?
Thrombin
Prothrombin
Factor VIII
Von Willebrand factorVon Willebrand factorWhich factor is activated as a result of the formation of coagulation complexes?
Factor X
Factor IX
Factor VIII
Factor IIFactor XWhich of the following is involved in the breakdown of the clot?
Tissue plasminogen activator
D-Dimers
Tissue factor
ThrombinTissue plasminogen activatorWhich anticoagulant is preferred for platelet testing?
Sodium citrate
Heparin
EDTA
Ammonium oxalateEDTAWhich is the preferred anticoagulant for most plasma assays of coagulation?
Heparin
Ammonium oxalate
EDTA
Sodium citrateSodium citrateWhat type of technology is used in the Coag Dx™ analyzer to evaluate coagulation?
LED detector
Collagen-coated membrane
Mechanical
Wire movementLED detectorWhat type of technology is used in the PFA-100 analyzer to evaluate coagulation?
Wire movement
Mechanical
Collagen-coated membrane
LED detectorCollagen-coated membraneWhich term describes the average size of the individual platelets in a sample?
Plateletcrit
Platelet-large cell ratio
Platelet distribution width
Mean platelet volumeMean platelet volumeWhich term refers to the percentage of the total blood volume that is comprised of platelets?
Platelet-large cell ratio
Platelet distribution width
Mean platelet volume
PlateletcritPlateletcritWhich test evaluates the variability in the size of platelets?
Platelet-large cell ratio
Platelet distribution width
Plateletcrit
Mean platelet volumePlatelet distribution widthWhich test provides a measure of the percentage of platelets that are larger than normal?
Platelet distribution width
Plateletcrit
Platelet-large cell ratio
Mean platelet volumePlatelet-large cell ratioWhich term describes newly released platelets that contain high levels of RNA?
Reticulated platelets
Platelet bands
RNA-rich platelets
Granulated plateletsReticulated plateletsWhich coagulation test uses a tube containing diatomaceous earth?
ACT
Bleeding time
PT
APTTACTWhich coagulation test evaluates the extrinsic coagulation pathway?
APTT
PT
Bleeding time
ACTPTWhat are D-Dimer and FDP tests used to evaluate?
Anticoagulant rodenticide toxicity
Secondary (chemical) hemostasis
Tertiary hemostasis
Primary (mechanical) hemostasisTertiary hemostasisWhat is the PIVKA test used to evaluate?
Primary (mechanical) hemostasis
Anticoagulant rodenticide toxicity
Secondary (chemical) hemostasis
Tertiary hemostasisAnticoagulant rodenticide toxicityWhich test represents a primary assay for the evaluation of platelet number and function?
Lee-White bleeding time
Platelet estimate
Buccal mucosa bleeding time
Clot retractionBuccal mucosa bleeding timeWhich coagulation assay is a good screening test for rodenticide ingestion?
Prothrombin time
Activated partial thromboplastin time
Von Willebrand antigen assay
Platelet countProthrombin timeWhat is the most common inherited coagulation disorder of domestic animals?
Thrombocytopenia
Hemophilia
Anticoagulant rodenticide toxicity
Von Willebrand diseaseVon Willebrand diseaseWhich term refers to the presence of pinpoint hemorrhage?
Purpura
Petechia
Ecchymoses
EpistaxisPetechiaWhich of the following is often found on a blood smear from patients with DIC?
Myeloproliferation
Schistocytes
FDPs
PolycythemiaSchistocytesDeficient or defective production of which coagulation factor results in hemophilia A?
Factor VII
Prothrombin
Christmas factor
Factor VIIIFactor VIIIWhat is the most common coagulation disorder of domestic animals?
Hemophilia
Thrombocytopenia
Anticoagulant rodenticide toxicity
Von Willebrand diseaseThrombocytopeniaWhich of the following are the vitamin-K-dependent coagulation factors?
Factors II, VIII, XI, and XII
Factors I, VII, X, and XII
Factors IV, IX, X, and XX
Factors II, VII, IX, and XFactors II, VII, IX, and XWhat is the primary site for the production of coagulation factors?
Liver
Bone marrow
Bile duct
SpleenLiverThe type of hypersensitivity reaction mediated by IgE is:
Type I
Type II
Type III
Type IVType IThe type of hypersensitivity reaction mediated by T cells is:
Type IV
Type I
Type II
Type IIIType IVImmune complex disorders are __________ hypersensitivity disorders.
Type III
Type I
Type II
Type IVType IIIAtopy is an example of __________ hypersensitivity.
Type I
Type II
Type III
Type IVType IAntibodies are produced by:
B lymphocytes
Neutrophils
Complement
T lymphocytesB lymphocytesThe cell that can differentiate into a macrophage when in tissue spaces is the:
Monocyte
T lymphocyte
Neutrophil
BasophilMonocyteWhich of the following is a component of adaptive immunity?
Complement
Hydrochloric acid
Skin
LysozymesComplementWhich leukocytes are involved in both the adaptive and nonadaptive immune systems?
Neutrophils
Lymphocytes
Eosinophils
BasophilsNeutrophilsSubstances that are capable of generating a response from the immune system are referred to as:
Antigens
Antibodies
NK cells
TNF cellsAntigensAn antigen-antibody reaction that results in the formation of insoluble particles is:
Precipitation
ELISA
Colloidal gold
OpsonizationPrecipitationThe primary immunoglobulin involved in mucosal immunity is:
IgA
IgG
IgM
IgEIgAA test that yields a negative result on a sample that is in fact negative is said to be highly:
Specific
Predictive
Precise
SensitiveSpecificThe term __________ refers to a test that has the ability to yield positive results on a sample that is in fact positive.
Sensitivity
Predictive value
Specificity
PrecisionSensitivityThe most common type of immunologic test performed in practice is:
ELISA
Agglutination
Immunodiffusion
Colloidal goldELISAFor the detection of a specific antigen, the solid phase of an ELISA test contains:
Antibody
Chromagen
Enzyme
ControlAntibodyRapid immunomigration is also referred to as:
Lateral flow assay
Radialimmunodiffusion
Latex agglutination
ELISALateral flow assayThe most serious transfusion reactions in canine patients occur in dogs that are positive for __________ and that are given mismatched transfusions.
DEA 1.1
DEA 3
DEA
MikThe vast majority of cats in the United States have type _____ blood.
A
AB
B
DalA major crossmatch involves the mixing of:
Recipient serum and donor cells
Recipient serum and donor serum
Donor cells and recipient cells
Donor serum and recipient cellsRecipient serum and donor cellsAn agglutination reaction with evidence of many small agglutinates and some 1
2
3
41The primary method of blood typing in reference laboratories is:
The tube method
Agglutination
Immunochromatography
CrossmatchingThe tube methodCanine agglutination blood typing is used to determine whether dogs are positive for:
DEA 1.1
DEA 3
DEA
MikDEA 1.1Neonatal isoerythrolysis has been documented in kittens of type _____ queens.
B
A
AB
MikBThe card agglutination assay for blood typing requires a sample collected with:
EDTA
No anticoagulant
Acid-citrate-dextrose anticoagulant
HeparinEDTAA minor crossmatch involves the mixing of:
Donor serum and recipient cells
Donor cells and recipient cells
Recipient serum and donor serum
Recipient serum and donor cellsDonor serum and recipient cellsThe most serious transfusion reactions in feline patients occur in cats with type _____ blood that are given mismatched transfusions.
B
Mik
AB
ABAllergies are mediated by:
IgE
IgM
IgA
IgGIgEA term that can be used to describe hives is:
Urticaria
Angioedema
Erythema
PetechialUrticariaA condition that involves swelling of the dermis is:
Angioedema
Urticaria
Erythema
PetechiaAngioedemaTest sites for intradermal testing should be at least __________ apart from each other.
2 centimeters
2 inches
1 centimeter
1 inch2 centimetersThe positive control for the intradermal test is given a grade of:
+4
+6
+2
0+4The Coombs tests is used to diagnose:
Immune-mediated hemolytic anemia
Combined immunodeficiency
Systemic lupus erythematosus
Feline infectious peritonitisImmune-mediated hemolytic anemiaWith regard to antibody titers, which statement is most accurate?
The higher the dilution, the more antibody in the sample.
Results are reported as either positive or negative.
A single, high-dilution titer (i.e., 1 : 160 or more) is indicative of an active infection.
The higher the dilution, the harder the immunologist had to look to find antibody in the sample.The higher the dilution, the more antibody in the sample.A type of immunologic test that is commonly used to detect and diagnose brucellosis is:
Agglutination
Colloidal gold
Neutralization
PrecipitationAgglutinationThe Coombs test is an example of a(n) __________ test.
Fluorescent antibody
Immunodiffusion
Radial immunodiffusion
NeutralizationFluorescent antibodyThe __________ test involves the addition of a sample to a plate coated with agar gel.
Immunodiffusion
Fluorescent antibody
Radial immunodiffusion
NeutralizationImmunodiffusion
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Env Geol Final
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