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Red tube additive is:


Red tube used for:

Chemistries, immunology , serology & blood bank cross match

Light Blue tube additive is:

Sodium citrate (Na citrate)

Light Blue tube used for:

Coagulation tests (PT, PTT, TCT, CMV) tube must be filled 100%

Light Green tube additive is:

Plasma separating tube (Na heparin)

Light Green tube used for:


Orange tube additive is:


Orange tube used for:

STAT serum chemistries

Dark Green tube additive is:

Sodium heparin or lithium heparin

Dark Green tube used for:

Ammonia, lactate, HLA typing For lithium level, use sodium heparin. For ammonia level, use sodium or lithium heparin

Gray tube additive is:

Sodium fluoride and potassium oxalate

Gray tube used for:

For lithium level, use sodium heparin. Glucoses requires a full draw (may cause hemolysis if short dray)

Red/gray "tiger" tube additive is:

Serum Separating Tube (SST) with clot activator

Red/gray "tiger" tube used for:

Blood type screening and chemistries

Gold tube additive is:

Separating gel and clot activator

Gold tube used for:

Serology, endocrine, immunology, including HIV

Royal or Dark Blue tube additive is:

Sodium heparin or Na2 EDTA

Royal or Dark Blue tube used for:

Toxicology and trace element testing (zinc, copper, lead, mercury) and drug level testing.

Yellow tube additive is:

ACT (acid-citrate-dextrose)

Yellow tube used for:

Paternity testing, DNA studies

Purple or Lavender tube additive is:

EDTA (liquid form)

Purple or Lavender tube used for:

Hematology (CBC) and Blood bank (crossmatch); requires a full draw- invert 8 times to prevent clotting and platelet clumping.

Brown tube additive is:

Sodium heparin

Brown tube used for:

Serum lead determination

Black tube additive is:

Sodium citrate (buffered)

Black tube used for:

Westergren sedimentation rate; requires a full draw.

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