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what's the difference between a prototroph and autotroph?
prototrophs have a wild-type complement of genes enabling them to grow with a simple nutrient requirement while auxotrophs are mutants unable to synthesize some small molecule on their own
what does an insertion sequence code for?
what flanks the transposase sequence?
what's the word for two insertion sequences flanking additional DNA like a resistance gene?
in lambda phage lysogenic infection, what is the name of the sequence that allows for recombination into the host chromosome?
what is a lysogen?
bacteria with viral DNA integrated into its chromosome
what do diphtheria, e coli, cholera and botulinum all have in common?
they house lysogenic phages that produce toxins
what are the two things that can happen to lysogenic phage when you treat with quinolone?
induce the promoter to synthesize toxin (shiga for example) and 2) transition to lystic phase (DON'T TREAT EHEC with QUINOLONE)
Name a con of phage therapy?
antibodies targeted against them will eventually reduce effectiveness
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