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what is the term for transfer of bacterial DNA from one cell to another by mispackaged phage?
how can you select for a transductant using generalized phase transduction?
insert an antibiotic resistance gene next to it and give it to transducing phages (odds are one of them will take up your piece)
what occurs when a lysogenic phage loops out of the bacterial chromosome and package up regions of the bacterial DNA only located right next to the phage insertion site?
specialized phage transduction
what is a high frequency of recombination cell?
an F+ cell that gets the F plamid incorporated into its chromosome (instead of just transferring the plamid you can not get transfer of a significant part of the chromosome... the conjugation bridge often isn't stable enough to transfer the whole chromosome)
Is conjugation limited by species?
nope (just more frequent between similar species)
What's the difference between natural competance and artificially induced competence?
refers to the ability to take up DNA from the environment. Artificially induced cells need treatment with calcium and magnesium salts or electroporation to do it
what is the most frequent method of horizontal gene transfer?
what type of recombination does REC-A mediate?
homologous recombination (binds ss region and invades into donor dna to match up homologous sequences)
what enzyme mediates site specific recombination? such as in lambda phage
integrase (small sequences are sufficient 3-5bp... doesn't need Rec-A)
which needs a larger region of dna: homologous or site specific recombination?
what is a the term for an operator, promoter, and one or more genes transcribed as a single polycistronic mRNA?
What is LacI in the lac operon?
Repressor (shuts off transcription in the absence of lactose)
what is lacy?
what is CRP?
positive regulator (activated by cAMP when glucose is low... binds to the promoter to stimulate transcription of the lac operon, but only works when lactose is around to remove lacI)
what is the difference between transversion and transition?
transversion is going from pyrimadine to purine while transition is purine to purine
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