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9 terms

Tissue Culture and GMO's

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micropropagation
propagating plants by the use of a small piece of tissue
Why use micropropagation?
can quickly grow many plants from very little plant material
obtaining explants
generally taken from meristematic tissue: flower, leaf, root, seed, stem
cleaning and treatment of explant
remove pathogens (heat treatment for viruses life fever in humans)
explant then transfered to media under sterile conditions, media contains...
nutrition, hormones, agar
transgenic recombinant plants
transformation; add one or more genes to a plants genome; gene isolation; performed in culture by use of Agrobacterium
Agrobacterium
is a genus of Gram-negative bacteria that uses horizontal gene transfer to cause tumors in plants.
genetically modified organisms (GMOs)
an organism whose genetic material has been altered using genetic engineering techniques. These techniques, generally known as recombinant DNA technology, use DNA molecules from different sources, which are combined into one molecule to create a new set of genes. This DNA is then transferred into an organism, giving it modified or novel genes
examples of GMOs
Bt Corn (insect resistance); Round-Up Ready Crops (glyphosate tolerance); Golden Rice (beta carotene-- bioavailability of iron, zinc, Vit A, Vit E)