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propagating plants by the use of a small piece of tissue

Why use micropropagation?

can quickly grow many plants from very little plant material

obtaining explants

generally taken from meristematic tissue: flower, leaf, root, seed, stem

cleaning and treatment of explant

remove pathogens (heat treatment for viruses life fever in humans)

explant then transfered to media under sterile conditions, media contains...

nutrition, hormones, agar

transgenic recombinant plants

transformation; add one or more genes to a plants genome; gene isolation; performed in culture by use of Agrobacterium


is a genus of Gram-negative bacteria that uses horizontal gene transfer to cause tumors in plants.

genetically modified organisms (GMOs)

an organism whose genetic material has been altered using genetic engineering techniques. These techniques, generally known as recombinant DNA technology, use DNA molecules from different sources, which are combined into one molecule to create a new set of genes. This DNA is then transferred into an organism, giving it modified or novel genes

examples of GMOs

Bt Corn (insect resistance); Round-Up Ready Crops (glyphosate tolerance); Golden Rice (beta carotene-- bioavailability of iron, zinc, Vit A, Vit E)

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