How can we help?

You can also find more resources in our Help Center.

41 terms

Microbiology Biochemical Test

STUDY
PLAY
Red
What is a color is the positive reaction for the Indole test?
E.coli
What organism tests positive for Indole?
Red
What color is a positive reaction for the Methyl Red test?
E.coli
What organism tests positive for Methyl Red test?
Gas bubble, orange/red/A+B, no color change after A+B+zinc
What is a positive reaction for a nitrate test?
Blue
What color is the positve reaction for Citrate Test?
Enterobacter
What organism tests positive for Citrate?
Liquid
What is a positive reaction for the Gelatin Test?
Bacillus
What organism tests positive for gelatin?
Clearing around the plate on addition of iodine
What is a positive reaction for the starch test?
Bacillus
What organism tests positive for starch?
More than 1/2 slant black
What's a positve reaction for the bile esculin test?
Enterococcus fecalis
What organism tests positive for bile esculine?
Purple with mineral oil
What is a positive reaction for the amino acid and decarboxylation test?
No mineral oil
What will make the amino acid test invalid?
yellow gas, yellow
What's a positive test for the carbohydrate/lactos and mannitol?
Clearing in agar around colony
What's a positive test for Casein?
Bacillus
What organism tests positive for casein?
Green
What's a positive test for PA (phenylalainine deaminase?)
Proteus
What's an organism that tets positive for PA?
Sexy pink
What's a positive test for urea hydrolysis?
Proteus
What organism tests positive for urea hydrolysis?
Fecal coliforms
What organimss are the MacConkey test looking for?
S. aureus
What organism Mannitol Salt Agar selective and differential for?
Salt content
What makes MSA selective?
Mannitol fermentation
What makes MSA differential?
The dyes in the media
What makes EMB selective?
Iridescent metallic green, black colonories
What makes EMB diffential?
Fecal coliforms, e.coli
What is EMB selective for?
Fecal coliforms
What is MAC looking for
lactose/carbohydrate fermentation
What makes MAC differential?
Crystal dye and bile salts
What makes MAC selective?
30 to 300
What are the only acceptable counts for Standard Plate Count?
Aerobic
For the aerotolerance test, if an organism grows only on top what is it considered?
Facultative
For the aerotolerance test, if an organism grows throughout, what is it considered?
An aerobe
For aerotolerance test, if an organism grows on the bottom what is it considered?
Fecal coliforms to detect water potability
What does the MPN test look for?
Yellow with oil and without oil
What is a fermantative organism look like in the OF test?
Yes
Does lactose broth contain e.coli? Acid + gas
No
Does lactose broth contain e.coli? Acid + no gas
S. aureus
Which organism ferments mannitol?