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5 Written questions

5 Matching questions

  1. What is debranching?
  2. What is glycogenin?
  3. Why is branching important in glycogen synthesis?
  4. What does alpha 1,6 glucosidase do?
  5. What are the properties of branching?
  1. a When glucose residues are removed to the "limit dextran" which is the 4th glucose residue from the branch glucose
  2. b Glycogenin is both an enzyme and its substrate consisting of two 37 kD subunits. Each subunit catalyzes addition of a glycosyl unit from UDP glucose to Tyr 194 of other subunit. Subsequently, 7 more glycosyl units are added so that 8 glycosyl units are attached to Tyr 194 for each subunit
  3. c Increases the number of terminal glucose residues which are site of action for glycogen phosphorylase and synthase. Branching increases rate of glycogen synthesis and degradation
  4. d Utilizes a non branched string of at least 11 glycosyl residues long. Branching enzyme transfers a string of typically 7 residues from non reducing end to presumptive branch point.
  5. e Alpha 1,6 glucosiase hydrolyzes the alpha 1,6 glucosidic bond to release a free glucose molecule

5 Multiple choice questions

  1. A very large, branched polymer of glucose residues. Most of the glucose residues are linked by alpha 1,4 glycosidic bonds. branches about every 10th residue are linked by alpha 1,6 glycosidic bonds
  2. UDP-glucose is synthesized from Uridine triphosphate and glucose-1-phosphate via the enzyme UDP-glucose pyrophosphorylase
  3. PPi+ H2O=> 2Pi
  4. Uridine diphosphate is a nucleotide composed of uracil, ribose and pyrophosphate
  5. Glucose-1-phosphate +UTP =>UDP-glucose + PPi

5 True/False questions

  1. What is the limit dextran?The 4th glucose residue from the branch glucose

          

  2. What enzymes are involved in Glycogen degradation?A glycosyl unit from UDP glucose is transferred to the hydroxyl group at the C4 of the terminal glucose residue forming a alpha 1,4 glycosidic bond. Requires a glycogen molecule that is a minimum of 4 residues to which a glycosyl will be added

          

  3. What are the properties of Glycogen synthase?A glycosyl unit from UDP glucose is transferred to the hydroxyl group at the C4 of the terminal glucose residue forming a alpha 1,4 glycosidic bond. Requires a glycogen molecule that is a minimum of 4 residues to which a glycosyl will be added

          

  4. What role does UDP-glucose play in Glycogen synthesis?A glycosyl unit from UDP glucose is transferred to the hydroxyl group at the C4 of the terminal glucose residue forming a alpha 1,4 glycosidic bond. Requires a glycogen molecule that is a minimum of 4 residues to which a glycosyl will be added

          

  5. What is the overall reaction of glycogen phosphorylase activity?Glucose-1-phosphate +UTP =>UDP-glucose + PPi