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5 Written questions

5 Matching questions

  1. What enzymes are involved in Glycogen degradation?
  2. What is the overall reaction of glycogen phosphorylase activity?
  3. What is glycogen?
  4. What is debranching?
  5. What does transferase do?
  1. a A very large, branched polymer of glucose residues. Most of the glucose residues are linked by alpha 1,4 glycosidic bonds. branches about every 10th residue are linked by alpha 1,6 glycosidic bonds
  2. b Transferase transfers the 3 terminal glucose residues from outer branch to the center, main linear branch
  3. c Glycogen phosphorylase, Transferase, alpha 1,6 glucosidase, phosphoglucomutase
  4. d Glycogen (n residues) + Pi=> glucose-1-phosphate + Glycogen (n-1 residues)
  5. e When glucose residues are removed to the "limit dextran" which is the 4th glucose residue from the branch glucose

5 Multiple choice questions

  1. Glycogenin is both an enzyme and its substrate consisting of two 37 kD subunits. Each subunit catalyzes addition of a glycosyl unit from UDP glucose to Tyr 194 of other subunit. Subsequently, 7 more glycosyl units are added so that 8 glycosyl units are attached to Tyr 194 for each subunit
  2. UDP-glucose is synthesized from Uridine triphosphate and glucose-1-phosphate via the enzyme UDP-glucose pyrophosphorylase
  3. Phosphoglucomutase is a phospho enzyme and converts Glucose 1 phosphate to glucose 6 phosphate for further metabolism
  4. Glucose-1-phosphate +UTP =>UDP-glucose + PPi
  5. Glycogen synthase catalyzes transfer of glucose from UDP-glucose to a growing chain

5 True/False questions

  1. What are the properties of the presumptive branch point?Utilizes a non branched string of at least 11 glycosyl residues long. Branching enzyme transfers a string of typically 7 residues from non reducing end to presumptive branch point.

          

  2. What are the properties of Glycogen synthase?A glycosyl unit from UDP glucose is transferred to the hydroxyl group at the C4 of the terminal glucose residue forming a alpha 1,4 glycosidic bond. Requires a glycogen molecule that is a minimum of 4 residues to which a glycosyl will be added

          

  3. What is Uridine Diphosphate?Uridine diphosphate is a nucleotide composed of uracil, ribose and pyrophosphate

          

  4. What is the limit dextran?A very large, branched polymer of glucose residues. Most of the glucose residues are linked by alpha 1,4 glycosidic bonds. branches about every 10th residue are linked by alpha 1,6 glycosidic bonds

          

  5. Why is branching important in glycogen synthesis?A glycosyl unit from UDP glucose is transferred to the hydroxyl group at the C4 of the terminal glucose residue forming a alpha 1,4 glycosidic bond. Requires a glycogen molecule that is a minimum of 4 residues to which a glycosyl will be added