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In experiments carried out in 1941 to investigate the citric acid cycle, oxaloacetate labeled with in the carboxyl carbon atom farthest from the keto group was introduced into an active preparation of mitochondria.
Analysis of the -ketoglutarate formed showed that none of the radioactive label had been lost. Decarboxylation of aketoglutarate then yielded succinate devoid of radioactivity. All of the label was in the released . Why were the early investigators of the citric acid cycle surprised that all of the label emerged in the ?
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