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17 terms

The 3 steps to DNA replication

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What is the 1st step of DNA replication?
The double helix must "unwind"
What must be broken that is between the nitrogenous bases?
hydrogen bases
DNA helicase-
enzyme that seperates or "unzips" DNA
place where DNA splits?
replication fork
What is the 2nd step of DNA replication?
nucleotides must be added in the correct order to create a complentary strand
primase-
enzyme that adds RNA primers so nucleotides can be attached
DNA polymerase-
enzyme that adds nucleotides (and proof reads, and replaces RNA primers with DNA nucleotides)
DNA polymerase can only read DNA in the ________ direction
3' --> 5'
it synthesizes a new complementary strand ____
'5 --> 3'
What is the 3rd step of DNA replication?
new strand must be sealed up
DNA ligase-
enzyme that seals the phosphate-sugar backbone of DNA together
what was the problem that okazaki solved?
DNA polymerase can only read DNA in the 3' --> 5' direction, it synthesizes a new complementary strand '5 --> 3'
The _____________ proceeds without difficulty because DNA polymerase can read it
leading strand
The _____________ must proceed in chunks, called okazaki fragments
lagging strand
prokaryote DNA replication-
DNA replication starts in 1 place continues in 2 different directions until the chromosome is copied
What is a chromosome also called?
plasmid
Eukaryote DNA replication-
DNA replication occurs at many places at the same time