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Exams 1,2,3 ONLY!

Excessive urination during the night


Marked increase in urine flow


A marked decrease in urine flow


complete stoppage of urine flow


A urine specimen is left on the urinalysis counter for 4 hours before being tested. Ketones would be


the fluid leaving the glomerulus has a specific gravity of

1.010 (pH of 7.4)

What substance are and are not reabsorbed in the ascending Loop of Henle

The ascending will reabsorb Na+ and Cl- and not water

what is the significance of a positive urine glucose result and a normal blood glucose

Renal tubular damage

The extent to which a measurement agrees with the true value of the analyte being measures is known as


The normal color of urine is produce by


the renal threshold is best described as the

concentration at which substance in the blood spills into the urine

What is the functional unit of the kidney

nephron (1-1.5 million in each kidney)

A urine specimen is left on the urinalysis counter for 4 hours before being tested. RBCs and WBCs would be


The primary site of reabsorption of glomerular filtrate is the

proximal convoluted tubule

to establish and maintain a quality control program in a laboratory with regards to procedures, each specific test should be


A specimen with a specific gravity of 1.005 would be considered

hyposthenuric (1.010-Isosthenuric, above 1.010 hypersthenuric, diluted urine below 1.010 is considered hyposthenuric)

Collected by passing a sterile tube into the bladder

catheterized specimen

Used to determine prostatic infection

three-glass collection

second voided specimen urine specimen collected after fasting

fasting specimen

specimen collected in a sterile container after cleansing the glans penis or urinary meatus

midstream clean catch specimen

Which of the following: Hydrometry, Urine reagent strips, freezing point depression, refractometry is not a standard method for measuring the specific gravity of urine

freezing point depression

A urine specimen is left on the urinalysis counter for 4 hours before being tested. Bilirubin would be


what requires a chain of custody

urine drug screen

a urine specimen is left on the urinalysis counter for more than 4 hours before being tested glucose would be


Normal urine is primarily composed of

water, urea, and sodium chloride

a urine specimen is left on the urinalysis counter for 4 hours before being tested. pH would be


Not permitting trash and combustible material to pile up, good housekeeping and keeping your work place neat and orderly can help prevent


What substances are and are not reabsorbed in the descending Loop of Henle

the decending will absorb water and not Na+ and Cl-

Fred Damon is at the lab to pick up a collection container for a 24 hour urine collection. What collection instructions would you give to Fred? Make sure you include storage requirements

Fred should Wake up in the morning and empty is bladder.
He should collect ALL urine for the next 24 hours
The next morning at the same time he should collect the last urine
During the collection Fred should keep the sample refrigerated

To extinguish a Class B fire you can use

an ABC fire extinguisher

When shaken, a urine specimen has yellow foam; what is most likely present?

protein and bilirubin

Complete the following creatinine clearance:
24 hour urine volume= 798 mL
Urine Creatinine= 120 mg/dL
Serum Creatinine= 2.3 mg/dL

Creatinine Clearance = (120 X 798)/(2.3 X1440)
Creatinine Clearance= 28.9 ml/Min

A urine specimen is left on the urinalysis counter for 4 hours before being tested. Bacteria would be


Safety regulations in the clinical laboratory fall under which government agency


You receive a urine for a urine drug screen that has a specific gravity of 1.000. What should you do? Make sure you include other tests that may be helpful?

This specimen is most likely not urine and should be rejected. Other test that would be helpful in confirming this would be creatinine and BUN. The urine temperature would also help determine if this was a true specimen.

What is the preferred method for urine preservation


Which of the following statements about Standard Precautions is correct?
A) Standard Precautions only applies to body fluids that may contain blood.
B) Standard Precautions applies only to blood.
C) Standard Precautions mean that all blood and body fluids should be handled as if they were infectious and capable of transmitting disease.
D) Standard Precautions do not apply to pleural fluid or semen.

Standard Precautions mean that all blood and body fluids should be handled as if they were infectious and capable of transmitting disease.

A urine specimen is left on the ruinalysis counter for 4 hours before being tested clarity would be


Color and it's significance:
Brown Black-Dark yellow-red-green

Brown black-melanin
Dark yellow-concentrated specimen
Green-pseudomonas infection

A urine concentration test was performed on a patient after a food and water deprivation period with the following findings:
After 14 hours Urine Osmolality = 850 mOsm/Kg
After 16 hours Urine Osmolality = 850 mOsm/Kg
What do these results indicate?

this represents loss of tubular function and concentrating ability

Freshly voided normal urine is usually clear; however, if it is alkaline, a white turbidity may be present due to

amorphous phosphates

what is the normal ratio of urine to plasma osmolality with controlled fluid intake


A patient has a sudden drop in blood pressure. The sequence of reactions in the RAAS is

renin, angiotensinogen, angiotensin I, angiotensin II, aldosterone.

What substances are not allowed to filter through the glomerulus in significant amounts?

Protein and cells

what can be used to confirm that a body fluid is urine


a urine specimen is left on the urinalysis counter for 4 hours before being tested. Nitrite would be


The principle of the reagent strip test for pH is

double indicator reaction

the purpose of scanning the perimeter of urine sediment placed under a conventional glass slide is to

detect the presence of casts

most renal calculi are composed of


the principle of the reagent strip test for protein is the

protein error of indicators

Oval fat bodies and fatty casts are characteristic urine sediment constituents in patients with

acute interstitial nephritis (When present in a urine specimen, these cells are accompanied by increased amounts of protein and cast formation. Oval fat bodies are often associated with nephritic syndrome)

Reagent strip chemical tests may be inaccurate if the specimen is

tested immediately after refrigeration

The presence of crenated RBCs in the urine sediment is associated with

hypersthenuria (Crenated RBCs are a sign of a hypertonic urine. The high specific gravity causes the water to leave the cells. Hypertonic urine can be seen in dehydrated patients or early morning specimens)

Differentiation among RBCs, yeast, and oil droplets may be accomplished by all of the following except

lysis of yeast cells by acetic acid (If the yeast cannot be differentiated on the basis of budding, 2% acetic acid can be added. RBCs will lyse; yeast will not. The oil droplets will have very defined refractile edges)

Phenylketonuria is caused by

lack of the enzyme phenylalanine hydroxylase

The type of protein that precipitates at 60F and dissolves at 100F is a/an

abnormal globulin associated with multiple myeloma (Bence Jones protein can be found in multiple myeloma. In multiple myeloma, the serum contains a high levels of immunoglobulin light chains or Bence Jones protein. Bence Jones protein can be detected by the heat soluble. Other proteins when heated will coagulate and remain coagulated. Bence Jones protein however will coagulate or precipitate at 60 degrees and will dissolve at 100 degrees. Serum electrophoresis should be done as a confirmatory test)

Phenylketonuria produces what kind of scent?


The recommended centrifugation for preparing the urine sediment is

400 RCF for 5 minutes

The location of epithelial cells in the urinary tract in ascending order is

squamous, transitional, renal tubular (The squamous epithelial cells are found in the urethra. Transitional can be found in the bladder and renal tubular in the kidneys)

The SSA test should be performed on

urine that has been centrifuged (The SSA test for protein is more sensitive the reagent strips and will detect other protein besides albumin. To perform the SSA a aliquot of 3% Sulfosalicylic acid is added to an equal amount of urine. The tube is mixed and graded from 1+-4+ based on the amount of precipitate. If a un centrifuged urine is used the turbidity from other element could interfere in this reading)

Acute renal failure may be classified by

post renal, pre-renal and renal

Sediment constituents that are used to differentiate between upper and lower urinary tract infections are

WBC casts (White blood cell cast are associated with pyelonephritis and infection. Since cast are only formed in the kidney, white blood cell cast pinpoint the location of the white blood cells and the infection to the kidneys. The white blood cells in the cast can be differentiated from red blood cells because they are larger and have multinuclei and granules in the cytoplasma. down)

To detect the presence of casts, the sediment is examined using

reduced light under low power (The casts will be reported out in number of cast per low power field. However high powered is used to differentiate the different types of casts)

Th presence of increased renal tubular epithelial cell casts is most indicative of

acute tubular necrosis

Cystinosis produces what kind of scent

a sulfur odor is associated with Cysinosis

Urobilinogen is formed from the

reduction of bilirubin by intestinal bacteria

the protein section of the reagent strip is most sensitive to


a spotted reaction on the reagent strip reaction for blood indicates


the cells most frequently associated with urinary tract infections are


motility by which of the following si most noticeable during the urine sediment examination

Trichomonas vaginalis

A 1+ reagent strip reaction and a 4+ Clinitest reaction could indicate the presence of

glucose and other reducing substances

Porphyrins are intermediary compounds in the formation of


Infection of the bladder is termed


the reagent strip reaction that should be read last is the

Leukocyte esterase

The substance producing the brown color in feces is


Normal crystals found acidic urine include

calcium oxalate, uric acid, amorphous urates

pediatric urine specimens are additionally tested for the presence of

galactose using Clinitest

Which of the following cells found in increased numbers in the urine sediment is only indicative of nephron damage

renal tubular cells

A medical technology student consistently obtains lower RBC counts than the instructor. A possible reason for this might be

failure to recognize crenated cells, focusing in the wrong plane, failure to centrifuge a mixed specimen

A false-positive test for 5-HIAA may result if the patient

does not receive appropriate diet instructions

A disease that causes large amounts of branched-chain amino acids to be excreted in the urine is

maple syrup urine disease

Inhibition of bacterial growth in the Guthrie test should be interpreted as

negative for PKU

the major constituent of casts is

Tamm-Horsfall protein

Excessive fat metabolism is indicated by the presence in the urine of


The normal range of pH is


Identification of crystals is based on shape and

urine pH and crystal size

The pseudoperoxidase reaction is the principle for the reagent strip test(s) for

both blood and creatinine

What can produce a negative nitrite test in the presence of significant bacteriuria?

production of nitrogen from the presence of many bacteria

The recommended diluting fluid for synovial fluid cell counts is

saline (WBC counts are most commonly done on a synovial fluid. If a fluid is very viscous hyaluronidase may need to be added. This will reduce the viscosity of the sample and allow the count to be performed. Many traditional WBC counting methods used acetic acid as a diluent but this would cause clot formation in a synovial fluid. To prevent this saline is used as a diluent)

The normal sperm concentration is

20-160 million/mL

the function of serous fluid is to

provide lubrication for the serous membranes

a dark green amniotic fluid is associated with


conditions the produce elevated CSF protein include all of the following except

fluid leakage (multiple sclerosis, hemorrhage, meningitis)

crystals found in the synovial fluid during attacks of gout are

monosodium urate

examination of a CSF shows 1000 WBCs, of which 75% are lymphocytes and 25% are monocytes this finding is consistent with

viral meningitis

exudate fluids usually result from

inflammation of the serous membrane

The pathological accumulation of fluid in a body cavity is termed a/an


a milky-appearing synovial fluid is associated with the presence of

monsodium urate crystals

A yellow color in semen specimen may be caused by all of the following except

preservation at room temperature

wet preparations for the detection of fecal neutrophils are stained with

Methylene blue ( Methylene blue is used for wet preps and Wright stain is used for fixed specimens)

When performing microscopic examination of a stool for muscle fibers, the structures that are counted

have vertical and horizontal striations

chemical analysis of CSF shows that the fluid contains

plasma chemicals in the concentrations different from those in the plasma

Stools from persons with steatorrhea contain excess amounts of


normal semen should liquefy

within 1 hour

Amniotic fluid tests for fetal maturity include

L/S ratio and microviscosity

Which one does not match:
A) Normal: clear, pale yellow
B) Traumatic tap: blood streaks
C) Crystals: milky
D) Sepsis: Uniform blood

Sepsis: Uniform blood

The term occult blood describes blood that

is not visibly apparent in the stool specimen

the normal CSF glucose is

60-70% of the blood glucose

before analysis semen specimens should be

allowed to liquefy

where is synovial fluid found?

Synovial fluid surrounds all joints, acts as a lubricant and supplies nourishment to cartilage
(It is NOT found only in the knee)

A xanthochromic CSF specimen will appear

yellow and clear

To determine the WBC count on a cloudy CSF specimen that contains both RBCs and WBCs, it is necessary to

dilute the specimen using glacial acetic acid

Semen analysis on postvasectomy patients should be performed

until two consecutive monthly specimens show no sperm

The third tube of CSF collected from a lumbar puncture should be used for

hematology tests

The presence of xanthochromia and an increased D-dimer test in the CSF is indicative of

a cerebral hemorrhage

Peritoneal fluid is collected by a procedure called


Which of the following pairings of stool appearance and cause does not match?
A) Pale, frothy: steatorrhea
B) Yellow-gray: bile duct obstruction
C) Black, tarry: blood
D) Yellow-green: barium sulfate

Yellow-green barium sulfate

The primary cause of decreased CSF glucose in bacterial meningitis is

alteration of blood-brain glucose transport

the normal brown color of the feces is produced by


spermatogenesis takes place in the

seminiferous tubules

Before and during collection of a sample for quantitative fecal fats, a patient should

consume 100 g of fat per day.

A CSF specimen, in which there is uneven distribution of blood among the three tubes and clots are detected, is an indication of

traumatic tap

A pleural fluid delivered to the laboratory in ice would be accompanied by a requisition to test for


Using a 1:20 dilution and the 5 RBC counting squares of the Neubauer counting chamber, an average of 54 sperm is counted. The sperm concentration is


The functions of the cerebrospinal fluid include all of the following except
A) protection of neurologic tissue from trauma.
B) removal of metabolic waste products.
C) transmission of neurologic impulses.
D) nutritional enrichment of nervous tissue

transmission of neurologic impulses.

Hemolytic disease of the newborn endangers the fetus by

destroying fetal RBCs (the fetus will suffer from anemia)

If CSF tubes numbered 2 and 3 cannot be analyzed within 1 hour, the correct procedure is to

refrigerate tube 3 and leave tube 2 at room temperature.

Tests for the detection of occult blood rely on the

pseudoperoxidase activity of hemoglobin

To determine if a questionable specimen is semen, the specimen should be tested for

acid phosphatase.

Crystals frequently seen with chronic inflammation are


Hemolytic disease of the newborn is caused by

maternal antibodies.

What is most often associated with the formation of a transudate?

Congestive heart failure (Transudates result from excessive filtration of blood serum across a physically intact vascular wall due to disruption of reabsorption. This occurs in systematic diseases that alter the hydrostatic pressure of the capillaries and include congestive heart failure, hepatic cirrhosis or nephritic syndrome)

To determine if an unknown fluid is synovial fluid, the fluid can be tested

by adding acetic acid and observing clot formation

A turbid synovial fluid with yellow-green color is indicates

infection (Specimens that are septic or caused by an infection can produce a specimen that is white, gray, yellow of green. The majority of infectious agents in septic arthritis are bacterial but can also be fungal or viral)

A milky, green-tinged pleural fluid

stains weakly with Sudan III

The recommended specimen for quantitative fecal testing is a

72-hour specimen

The foam, or shake, test is a screening test for amniotic fluid


An amniocentesis is performed on a woman whose last two pregnancies have resulted in stillbirths due to hemolytic disease of the newborn. A screening test performed at the hospital is positive for bilirubin, and the specimen is sent to a reference laboratory for a bilirubin scan. Doctors are concerned when the report comes back negative, and they question if the

specimen was exposed to light

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