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Chapter 9

Terms in this set (15)

Cold-reacting Antibodies (room temp or colder)
Lewis, M, N, P1, Lua, I
Albumin-enhanced (37 degrees) Antibodies
Rh and Kell Systems (occas. M)
AHG Antibodies -
Rh, Kell, Duffy, Kidd, Ss, Lub
In vitro Hemolysis -
anti-Lea, anti-Leb, anti-P1PPk, anti-Kidd, anti-Vel
Enzymes
ficin, bromelin, papain, trypsin
Modify RBC surface by removing sialic acid residues and denaturing or removing glycoproteins
enhance:
ABH, Rh, Lea and Leb, Jka and Jkb, P1, I, some Kell
destroy
M, N, S, Fya, Fyb, Fy6, Yta, Ch, Rg, Pr, JMH, Ge2, Xga
REST absorption
rabbit erythrocyte stroma (absorbs anti B).
-- used to absorb cold reacting antibodies.
Sulfhydryl Reagents
destroys IgM (cleaves intersubunit disulfide bonds, so destroys any antigen that would have extensively folded disulfide bonding)
Thiol - DTT (dithiolthreitol)
inactivates Kell antigens (except Kx), Kna, Yta, JMH, Hy
2ME (mercaptoethanol) -
similar activity to DTT
AET - 2 aminoethylisothiouronium bromide
inactivates Kell (except Kx), Yka, Yta, Hy, Kna, Lub
ZZAP
protease (papain) and thiol (DTT); reduced IgG and enzyme-treated makes cells suitable for autoabsorption
-- inactivates Kell (except Kx), M, N, S, Fya, Fyb, Fy6, Pr, Ch, Rg, Yta, Xga
CDP
chloroquine diphosphate
-- removes IgG from RBCs (+DAT) so cells can be typed
-- inactivates Bg antigens, some Rh antigens weakened
Acid Elution
used to remove warm-reactive IgG antibodies from RBCs; RBCs hemolyzed and antibodies salvaged in supernatant; citric acid, glycine, and digitonin acids can be used.
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