20) You are interested in understanding the gene regulation of a protein called LKP1, which is normally produced in liver and kidney cells in mice. Interestingly, you find that LKP1 is not expressed in heart cells. You replace the coding sequence for LKP1 with the DNA encoding green fluorescent protein (GFP) and examine the expression of GFP in mice. (GFP is your reporter gene.) You find expression of GFP in liver and kidney cells but not heart cells; this expression pattern is similar to how LKP1 is expressed normally. Further experiments demonstrate that there are three regulatory sequences in the promoter, labeled A, B, and C in Figure 8, that are important for this expression pattern. You want to determine the significance of each regulatory sequence, so you create situations where only a subset of regulatory regions are present upstream of the reporter gene, as diagrammed in Figure 8.
Given the data, which of the statements below is false?
A) Regulatory sequence A switches on LKP1 in the kidney.
B) Regulatory sequence B switches on LKP1 in the liver.
C) Neither regulatory sequence A nor regulatory sequence B is required for LKP1 expression in the heart.
D) Regulatory sequence C is not necessary for proper expression.
E) When regulatory sequences A and B are present, expression is in liver, kidney and heart.