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105 terms

Microbiology final exam

flashcards from lab reports
STUDY
PLAY
Ocular(eyepiece)
A series of lenses that usually magnify 10 times.
Revolving nosepiece
Rotates to change from one objective to another
Objective
The lens closest to the object
Diaphragm
Opens and closes with a lever controlling the amount of light striking the object
Condenser
Condenses light waves into a cone,thereby preventing escapes of light rays; raused and lowered to control the amount of light striking object.
Mechanical stage
allows the slide to move
base
supports the entire microscope
arm
supports upper portion of microscope
coarse adjustment
moves stage or body tube up and down rapidly for purposes of approximate focusing
fine adjustment
moves stage and body up and down very slowly for purposes of definitive focusing
body tube
raised and lowered in focusing in microscopes
transient bacteria
bacteria that could easily b removed from the skin
resident bacteria
bacteria that normally live on the skin
Ignatz Semmelweis
the man who first observed the relationship between handwashing and disease
Puerpal fever
The disease that help reduceby requiring medical student to wash their hands
Staphylococci
MSA medium is selective for
Yeast
maybe found in the resident microbe group.
Transient flora
mold spores may be part of the
Feces
typhoid fever may be spread by hands in contact with
False
A single antibiotic is usually toxic to both prokaryotes and eukaryotes.
small doses
antibiotic is usually effective in
cidal
means"kill"
static
means"inhibits growth,stop,to arrest"
antiseptics
agents used to kill or inhibit growth of vegetative cells are
sanitizers
agents uses to kill bacteria on well cleaned food handling equipment
Microbiology
the study of microorganism
Pathogenic
disease - causing
Parasitology:
study of protozoan and helminth caused disease
Antibiotics:
chemotherapeutic agents produced by microorganisms.
Chemotherapy:
treatment of disease with chemicals
Immunity
stimulating the body's own ability to combat infection
Immunology
study the immune system, which provides us protection against pathogen.
Bubonic plague
caused by Yersinia pestis, infected fleas carried the bacterium, bite the rat. Rat dies and fleas bite human. Made the lymph glands swollen
Buboes
Kiled 25 million people in Middle ages
Potato blight
fungus Phytophthora infestans infected the potato caused the starvation of Irish faminie in 1800s
Typhus
a bacterial disease, in 1812, caused Napoleon loss to Russia
small oox and measles
European brought the diseases to Central and south American
Why study microbiology?
Microorganisms are part of the human environment and are therefore important to human health and activities.
Environmental Microbiology ( microbiology ecology
the study of how microorganisms affect the earth and its atmosphere
Recyle vital elements (C,N,O,S,P )
convert elements into forms that are useful
sewage treatment
remove suspension and harmful microbes; recyle water
Bioremediation
use microbes to clean up pollutants (oil spills,etc).
HISTORICAL ROOTS
The ancient Greeks, Romans, and Jews all contributed to early understanding of the spread of disease.
Hooke(1665)
Defines the term 'cell', had his microscope
Anton van Leewenhoek
Build his own microscope, was the first to see microorganisms. He called them animalcules. kept his microscope to himself (no advancement for more than a century)
Louis Pasteur
with his swan-necked flasks, dust-free air, disproof spontaneous generation.- Pasteurization (work for the wine industry; heating wine to 56 degree in the absence of oxygen for 30 minutes) - germ theory of disease, microorganisms (germs) cause infectious diseases, and specific microorganisms cause specific diseases.
Robert Koch
- worked on anthrax developing technique to study the bacterium in vivo,grew bacteria in pure culture (use of agar),formulated the postulates,identified the bacillus that cause tuberculosis, developed tuberculin,and studied cholera
Koch's Postulates
1. Specific causative agent must be found in every case of the diseases.2.The diseases organism must be isolated in pure culture.3.Inoculation of a sample of the culture into a healthy, susceptible animal must produce the same disease.4The disease organism must be recovered from the inoculated animal.
Ignaz Phillip Semmelweiss
Austrian who set about controlling infections,Made connection between autopsies and puerperal (childbed) fever (Streptococcus pyrogens causing sepsis),Instead ridiculed, harassed; end up in the asylum
Lister (1865)
Use carbolic acid; his methods (the first asceptic techniques) proved effectively by the decrease in surgical wound infections in his surgical wards)
Paul Ehrlich
-chemotherapy,- introduced an arsenic compound and Salvarsan (effective against syphilis)
Alexander Fleming
accidental discovery of penicillin (antibiotics) from Penicillium notatum,lysozyme could kill bacteria
Martinus Beijerinck
Dutch microbiologists; first characterize viruses,2. Show recycling of elements by bacteria
Peyton Rous
Work on connection between viruses and cancer (transmission of chicken sarcoma)
Salk
worked on poliovirus, developed vaccine (1954),inactivated poliovirus (IPV) that required injections,currently used version to immunize children
Sabin
Work on poliovirus; developed an oral polio vaccine (OPV); contains living attenuated strains of virus; virus ingested, also excreted
Robert Gallo
Discover human immunodeficiency virus (HIV), the cause of AIDS in 1983.
Elie Metchnikoff
pioneer in immunology,discovered phagocytes and phagocytosis,developed several vaccines
Jenner - Immunology
-noticed milkmaids who came down with cowpox did not get smallpox,-introduced vaccination for small pox (Variola) in 1796. attenuated (weakened),-innoculated people with cowpox to protect against smallpox-
cocci
(sphere)
bacilli
(rod)
spirillum
(spiral)
vibrio
bend rod)
staphylo
clusters
strepto
chains
Viruses -
size, acellular, nuclei acid is DNA or RNA; obligate intracellular parasites,Virus can only produce in a host cells.Viruses are particles of nucleic acid (either RNA or DNA), usually enclosed in a protein coat and sometimes surrounded by a membrane.Viruses are extremely small, even compared with bacteria.Viruses can infect animals, plants, and microorganism.
viriods
small RNA pieces (folded) that infect plants; cause lot of crop damage; no protein coat
Prions
proteinaceous infectious particle; responsible for diseases such as BSE (Bovine Spongiform Eucephalopathy) - mad cow disease; kuru, CJD, fatal familial insomnia. One hypothesis on how abnormal prion proteins corrupt normal prion protein and tangled in brain.
protozoa
unicellular, eukaryotic, absorb or ingest food, reproduce sexually or asexually.Superficially animal - like, Amoebae move by pseudopods. Others use flagella or cilia to move. heterotroph with food vacules,nonphotosynthetic, and usually motile.
Examples of protozoa
are amoebae, flagellates, and ciliates
parasitology.
The study of protozoa (and helminth-caused) disease is called
Helminths
are macroscopic worms, but some go through microscopic stages in their life cycle; they cause parasitic diseases in plants and animals, including humans.The helminthes important to health studies are flatworms and roundworms.
eukaryotes
Algae, fungi, and protozoa are ,their organelle are membrane bound. can be single celled or multi-celled organisms
viruses
are acellular.
prokaryotes
they lack internal membrane-bound structures,Bacteria and archaea are and single celled.
Rickettsia
bacteria (gram negative; rod shaped) obligate intracellular parasites of mammalian cells
Cynobacteria
blue-greens; are bacteria and not algae; carry out photosynthesis; can fix nitrogen gas into ammonium using specialized cells called heterocysts
Morphologically varied
unicellular forms, filamentous, colonial forms
Bacteria(sing., bacterium)
unicellular, prokaryotic, presence of peptidoglycan in cell wall, antibiotic sensitivity.They can be spherical, rod-shaped, helical, comma-shaped, star-shaped or square. Heterotrophs or autotrophs.Bacteria are extremely small, even for microorganisms.Bacteria vary in shape, motility, and how they get energy. There are species that can withstand freezing, boiling, and extreme acidity or alkalinity.
Archaea (sing., archaeon)
- unicellular,prokaryotic, absence of peptidoglycan in cell wall, no sensitivity to antibiotic, Archaea were discovered as a separate group of microorganisms in the 1970s.At first, they were called archaebacteria.Being prokaryotes and small, they resemble bacteria superficially, but they are as distantly related to bacteria as they are to eukaryotes. At first, they were called archaebacteria.Being prokaryotes and small, they resemble bacteria superficially, but they are as distantly related to bacteria as they are to eukaryotes. At first, they were called archaebacteria.Being prokaryotes and small, they resemble bacteria superficially, but they are as distantly related to bacteria as they are to eukaryotes. At first, they were called archaebacteria.Being prokaryotes and small, they resemble bacteria superficially, but they are as distantly related to bacteria as they are to eukaryotes. At first, they were called archaebacteria.Being prokaryotes and small, they resemble bacteria superficially, but they are as distantly related to bacteria as they are to eukaryotes.
Algae(sing.,alga)
- photosynthetic eukaryotes (usually unicellular), cell wall of cellulose,Eukaryotic organism carry out plantlike photosynthesis. Make a big mass of phytoplankton., autotroph,Some algae are unicellular and microscopic. Others consist of many cells and are macroscopic.,Algae are not significant medically, but they are critically important to global ecology.
Fungi (sing., fungus)
Include mushroom, yeasts, and molds. Most of them are scaverngers - decompose the dead organisms. Eukaryotes, multicellular, heterotroph with absorptive nutrition: conidia/ hyphae
Mycelium
Fungi grows multibranched tubes called
The Microbiologists
Immunology, virology, chemotherapy, and genetics are especially active research fields of microbiology.Microbiologists work as researchers or teachers in university, clinical, and industrial settings. They do basic research in the biological sciences; help to perform or Microbiologists work as researchers or teachers in university, clinical, and industrial settings. They do basic research in the biological sciences; help to perfoem or
Domains
Archaea (ancient) Bacteria (eubacteria) Eukarya
Chromosome
The DNA molecule must contain genetic information essential for the continuous survival of the organism
Pro
before
Eu
true
karyon
nucleus
Acid-Fast Bacteria
Found in bacteria that belong to the genus, Mycobacterium sp.Cell wall is mainly composed of lipid,Lipid component is mycolic acid,and Acid-fast bacteria stain gram-positive
Plasmids
contain only genetic information that could be helpful to organisms, but that they could survive without
Mutation
Change in the genetic sequence of DNA in a cell which may or may not cause a change in the amino acid sequence coded from that section of DNA.
Protein genetic flow
DNA>polmerase>transcription>mRNA>Ribosome(protein+rRNA)>translation>tRNA+ amino acid>peptide bond>polypeptide>protein
Transcription
is the synthesis of mRNA from a DNA template
food
sterilizers are used only on.......utensils.
millimeter
zones of i nhibition are measured and recorded in millimeter
live
a disinfectant is expected to let endospores
animated
an antiseptic is usually used on ______objects
antiseptic
an agent used as a gargle would commonly be called__________
1bacteriostatic property(antibiotic) 2. used on animated objects (disinfectant)
list two differences between an antibiotic and disinfectant.
smear
a preparation made by mixing a loopful of water and a bit of agar slant culture on a glass slide
differential
the gram stain is an example of-----------stain.
negative
all members of the genus e.coli would be expected to be gram_
clear
endospores usually appear _____ in a completed gram stain.
decolorize
the most critical step in making the gram stain is the application