To seperate plasma from the cellular components, blood tubes should be counterbalanced and centrifuged for _____ mins at ______ rpm.
EDTA plasma cannot be used for testing ______ plasma levels because EDTA forms a complex with it.
Veterinary reference ranges for blood chemistry values
often come with an analyzer and have been established by the company
If a test result is a false positive, it means that the result is
outside the reference range and the disease is absent
Which of the following would not be an acceptable action if a control value is out of range?
ignore the result; it is a simple outlier (sporadic results)
Which of the samples can be frozen and successfully thawed for performing an analytic test at a later time?
serum for chemistry testing
If there is only a small amount of serum separated after centrifuging a tube of whole blood, you should
rim/ring the clot and spin again at normal speed and time
To dilute a serum sample for retesting when the value is out of the instrument's linearity range, use
Regarding the disadvantages of using commercial laboratories, which statement is false?
they often do not adhere to quality control standard
What is the difference between a red-top Vacutainer and one with a mottled red-and-black top?
one contains serum separator gel and the other does not
The hematology analyzer is not functioning properly. A good mathematical method to estimate hemoglobin is
one third the PCV
When collecting blood for a coagulation profile, it is especially important to
minimize vascular trauma during venipuncture
A box of reagents for blood chemistry analysis labeled for storage at 8* C should be stored
in the refrigerator
The red-top Vacutainer tube should sit at room temperature for ____ before centrifugation, allowing the clot to form.
If you are unable to perform a CBC within 1 hour of blood collection, which of the following is the best method of preservation?
Refrigerate the sample
If a 1:1 dilution of a serum sample is made before analyzing the serum chemistries, the results must be multiplied by
Which of the following statements is true in regard to using hemolyzed serum in a dry chemistry analyzer?
hemolyzed serum can falsely elevate some results
What information do the RBC indices yield?
the size, weight, and hemoglobin concentration of an average RBC
A new in-house test has been developed with 80% sensitivity and 100% specificity. What does this mean?
20% will be false negative
When the spun hematocrit test is done, which of the following can be evaluated?
total protein, buffy coat, plasma color, and PCV
Prolonged exposure of serum to the blood cells before the serum is removed from the clot can result in
increased serum phosphorus
When using a centrifuge, it is important to do the following each time you use it, except
open the lid as the centrifuge is stopping
As a general rule, enough blood should be collected to run any test at least three times. This compensates for all of the following, except
Ethylenediaminetetraacetic acid (EDTA) is the anticoagulant of choice for hematology because it
preserves blood cell morphology
What is the noncellular portion of coagulated whole blood called after is has been centrifuged?
What is the noncellular portion of anticoagulated whole blood called after it has been centrifuged?
When mailing serum samples to a reference laboratory, which of these would you not want to do?
mail samples out on Saturday
What is the disadvantage of using vacuum blood collection tubes?
withdrawal pressure is not easily controlled
Serum should be separated from blood cells in a blood sample as soon as possible because
some chemical constituents will change
What condition will not cause hemolysis?
removing the needle from the syringe to place a blood in a collection tube
Which of the following is not a consideration when choosing the gauge and length of a needle for blood sample collection?
test to be run
If whole blood is allowed to stand at room temperature for an extended time,
glucose levels may decrease
If you are unable to perform chemistry tests on a sample for a week, you should
centrifuge the sample, separate the serum, and freeze it
Diagnostic testing performed in a laboratory setting, outside of the animal's body, is
in vitro testing
Performing a group of blood chemistry tests to evaluate the overall health of an animal is
When plasma is required for blood chemistry analysis, the anticoagulant of choice is
sodium or lithium heparin
The anticoagulant used to preserve blood glucose levels for blood chemistry analysis is
As a general rule, sufficient blood should be collected from the animal to yield enough serum, plasma, or whole blood to run each test how many times?
To preserve blood constituents in the event that sample analysis is delayed for 8 hours, serum or plasma should be
refrigerated and warmed to room temperature before analysis
For most blood chemistry examinations, serum or plasma samples are stable at room temperature for
4 to 7 hours
For most blood chemistry examinations, serum or plasma samples are stable at refrigerated temperatures for
4 to 7 days
For most blood chemistry examinations, serum or plasma samples are stable at frozen temperatures for
4 to 7 months
Centrifuging blood that has been collected in a plain (red-top) vacuum tube and allowed to clot produces
False evaluations in potassium and inorganic phosphorus levels can be expected in a serum sample that is
Drawing a blood sample from an animal that has recently eaten may result in a sample that is
Which statement is false concerning collection of a plasma sample for blood chemistry analysis?
To separate, allow the sample to clot for approximately 30 minutes, gently remove the clot from the sides of the tube, and centrifuge and remove the plasma
What sample collection cannot be minimized by proper animal preparation or proper sample collection and handling?
The technology of dry chemistry differs from that of wet chemistry in that the reagents in dry chemistry are supplied
impregnated on slides, cards, or strips
In wet chemistry technology, the amount of chemical substance in the blood is determined by measuring the
light passing through or absorbed by the final sample
In dry chemistry technology, the amount of chemical substance in the blood is determined by measuring the
light reflected from the slide, card, or strip
To verify the accuracy of blood chemistry results in the laboratory, the technician should use
What condition will not affect the accuracy of a blood chemistry kinetic enzymatic assay?
final color change of the sample
How long must the typical microhematocrit tube be centrifuged for packed cell volume determination?
What anticoagulant is used most commonly in animal-blood collection for CBCs?
potassium ethylenediaminetetraacetic acid
Basophilic stippling may be seen in erythrocytes of patients with which of the following pathologic conditions?
The process of monitoring instruments, reagents, tests procedures, technician performance, and the accuracy of reported results is
The calibrated device used to deliver a specified volume of patient sample when performing blood chemistry analysis is a
The group of values for a particular blood constituent, derived when a lab has repeatedly assayed samples from a significant number of clinically normal animals of a given species, is called
the reference range
The rate of an enzymatic reaction is measured while it is in progress during what type of reactions?