Terms in this set (40)
In the lab, DNA molecules can be cut at specific sequences using
On average, which restriction nuclease would cut a random DNA sequence into a greater number of pieces?
a restrictive nuclease that recognizes four-nucleotide pair sequence
How does a bacterium protect its a DNA from cleavage by its own restrictive nucleases?
The bacterium chemically modifies its own DNA sequences thereby preventing recognition by the nucleases
Gel electrophoresis separates DNA fragments by virtue of
When a mixture of DNA fragments of different sizes is loaded onto an agarose gel and a voltage is applied, the DNA will migrate
the shortest the fastest
When DNA fragments are being separated by gel electrophoresis, toward which electrode do the fragments move?
the positive electrode
Incorporating radioactive isotopes into DNA prior to gel electrophoresis is one method to
visualize the separated DNA bands in the gel
Nucleic acid hybridization can detect any give DNA or RNA sequence in a mixture of nucleic acid fragments. This technique relies on the fact that
a single strand of DNA or RNA will form a double helix only with another nucleic acid strand of the complementary nucleotide sequence
In Southern blotting DNA fragments are first separated from each other by
What does the term DNA cloning refer to?
The act of making identical copies of a DNA molecule
Which enzyme allows scientists to join together two DNA fragments?
Which of the following statements is NOT true of a plasmid?
It inserts the gene of interest into the bacterial chromosome
DNA can be introduced into bacteria by a mechanism called
What does a DNA library consist of?
A collection of cloned DNA fragments in a bacterial culture
Which of the following DNA libraries lacks introns and regulatory sequences?
a cDNA library
When making a DNA library from a starting material of mRNA, which enzyme is required to copy the mRNA molecules into DNA molecules?
Which of the following libraries would be expected to be essentially the same?
genomic libraries made from mouse liver and kidney cells
What is the starting material for making a cDNA library?
If you want to clone a gene so that you may deduce the amino acid sequence of the protein from the DNA, what type of library would be the most useful to start from?
a cDNA library
Which is NOT true
PCR can be used to sequence a genome (this IS NOT true)
In order to design primers for the polymerase chain reaction, which part of the DNA sequence of interest must be known?
The ends of the DNA sequence of interest
In the polymerase chain reaction, what is used to separate the two strands of a double-stranded DNA molecule?
Using the procedure of polymerase chain reaction, after 30 rounds of replication and beginning with one template molecule of double-stranded DNA, how many copies of the target sequence would you expecT?
about 1 billion
What serves as the original template material for the polymerase chain reaction
DNA or RNA
In order to detect an infectious microorganism by PCR, what is required as a primer?
Short sequences complementary to a part of the microbe's genome
Sanger sequencing is used to:
simply and quickly determine the nucleotide sequence of any purified DNA fragment
All sequencing methods, including second- and third- generation sequencing methods, require DNA amplication
One relatively new, third-generation sequencing method allows:
sequencing of a single molecule of DNA by detecting the shape of its nucleotides
By comparing a nucleotide sequencing available tin public database, one CANNOT:
determine the gene's precise role in the physiology or development of the organism
Which of the following technologies allows us to study the expression patterns of thousands of genes at the same?
Which of the following techniques allows a researcher to identify the location of particular RNAs in a cell?
In situ hybridization
A reporter gene
reveals when a gene is expressed, reveals where a gene is expressed, and is a gene whose activity can be easily monitored
Which of the following is considered a reporter protein?
Green fluorescent protein
Which of the following refers to the appearance or behavior of an individual?
The "classical genetic approach" works best with organisms that reproduce rapidly and can be analyzed individually in the laboratory
Which of the following statements is true?
RNA interference is based on a natural mechanism many organisms use to avoid infection
Which of the following terms describes a mouse in which a particular gene has been genetically engineered?
Transgenic animals are used to model human diseases in which mutant genes play a major part because
transgenic approaches in humans are unethical
Plants are resistant to manipulation by recombinant DNA techniques
What extra sequences are found in expression vectors that are not found in typical cloning vectors?
Sequences that direct the production of large quantities of protein